中国临床医学2012，Vol.19Issue(3)：198-200,3.
系统性硬皮病患者外周血单个核细胞对皮肤克隆成纤维细胞胶原代谢的影响
Effects of Peripheral Blood Mononuclear Cells on the Collagen Metabolism of Cloned Systemic Scleroderma Skin Fibroblasts
摘要
Abstract
Objective: To study the effects of peripheral hlood mononuclear cells (PBMCs) on the collagen metabolism of cloned systemic scleroderma (SSc) skin fibroblasts with heterogeneity in the synthesis of collagen. Methods:By means of realtime reverse transcription-polymerase chain reaction (RT-PCR),the effects of PBMC supernatants (from SSc patients and controls) on the expression of type I procollagen and matrix metalloproteinase-1 (MMP-1) on mRNA level in cloned SSc skin fibroblasts with heterogeneity in the synthesis of collagen were determined. Results: Type I procollagen and MMP-1 mRNA levels of cloned SSc fibroblasts were upregulated by PBMC from SSc patients (10%,48 h) only in low-collagen-producing clones [(0. 70 ± 0. 29) vs. (0. 07 ± 0. 08),P = 0. 001; (0. 75 ± 0. 16) vs. (0. 09 ± 0. 20),P<0. 001]. Type I procollagen mRNA levels of cloned SSc fibroblasts were downregulated by PBMC from controls (10% > 48 h) only in high-collagen-producing clones [(-0. 24 ± 0. 08) vs. (0. 12 ± 0. 10),P = 0. 024]. MMP-1 mRNA levels of cloned SSc fibroblasts were upregulated by PBMC from controls(10%,48h) in both high and low-collagen-producing clones (P = 0. 003,P<0. 001),and the increase was greater in low-collagen-producing clones than in high-collagen-procuding clones [(1. 27 ± 0. 23) vs. (0. 56 ± 0. 08),P = 0. 001]. Conclusions; Type I procollagen and MMP-1 mRNA levels of cloned SSc skin fibroblasts with heterogeneity in the synthesis of collagen are heterogeneous in their response to PBMCs.关键词
系统性硬皮病/成纤维细胞/克隆细胞/胶原/外周血单个核细胞Key words
Systemic scleroderma/Fibroblasts/Clone cells/Collagen/Peripheral blood mononuclear cell分类
医药卫生引用本文复制引用
高地,朱鹭冰,李明..系统性硬皮病患者外周血单个核细胞对皮肤克隆成纤维细胞胶原代谢的影响[J].中国临床医学,2012,19(3):198-200,3.基金项目
国家自然科学基金(编号:30471561) （编号:30471561）
