中国临床医学2012，Vol.19Issue(3)：201-203,3.
脂多糖刺激黏液下腺细胞后黏蛋白5AC和水通道蛋白5的变化
Decreased Aquaporin-5 and Increased Mucin 5AC in SPC-A1 Cell Line Stimulated by Lipopolysaccharide
摘要
Abstract
Objective:To investigate the changes of aquaporin-5(AQP5) and mucin 5AC(MUC5AC) in SPC-A1 cell line stimu-lated by lipopolysaccharide (LPS). Methods: After SPC-A1 cell line was stimulated by LPS,the mRNA levels of AQP5 and MUC5AC were detected by real-time polymerase chain reaction (PCR),and AQP5 and MUC5AC protein were measured by Western blot and enzyme-linked immunosorbent assay (ELISA). Results; The protein expression and transcription of AQP5 decreased while the MUC5AC expression increased in a dose (0 μg/mL、5μg/mL、10 μg/mL,20μg/mL and 40μg/mL,respectively) and time-dependent manner by LPS stimulation in SPC-Al cell line. Conclusions: Decreased AQP5 expression was correlated with increase of MUC5AC in SPC-Al cell line after LPS exposure.关键词
黏蛋白5AC/水通道蛋白5/脂多糖/黏液下腺细肥Key words
Mucin 5AC/Aquaporin-5/Lipopolysaccharide/Submucosal gland cell分类
医药卫生引用本文复制引用
沈瑶,白春学,金美玲,陈智鸿..脂多糖刺激黏液下腺细胞后黏蛋白5AC和水通道蛋白5的变化[J].中国临床医学,2012,19(3):201-203,3.基金项目
国家自然基金(编号:81000012) （编号:81000012）
上海市重点学科建设项目(编号:B115) （编号:B115）
