中国临床医学2012，Vol.19Issue(3)：204-206,3.
肺炎衣原体诱发肺癌模型的建立
Study on Lung Cancer Model Induced by Chlamydia Pneumoniae
摘要
Abstract
Objective: To build a rat model of lung cancer induced by Chlamydia pneumoniae(Cpn) infection. Methods: Cpn (TW-183) was injected into the lungs of the rats with or without the combination of Benzopyrene(Bp). Cpn-antibodies(Cpn-IgA,-IgG and -IgM) in rat serum were detected by the method of microimmunofluorescence(MIF). The Cpn-DNA and Cpn-Ag in lung cancer tissues were detected by polymerase chain reaction(PCR)and enzyme-linked immunosorbent assay(ELISA),respectively. Results: The incidences of Cpn infection were 72. 9%(35/48) in rats injected with Cpn and 76. 7%(33/43) in rats injected with Cpn and Bp; and the incidences of lung cancer in them were 14. 6% (7/48) and 44. 2% (19/43),respectively,all of which were significantly higher than the controls(P = 0. 012,P<0. 001,respectively). Conclusions: A rat model of lung cancer induced by Chlamydia pneumoniae infection has been successfully built.关键词
肺炎衣原体/苯并芘/肺癌/动物模型Key words
Chlamydia pneumoniae/Benzopyrene/Lung cancer/Animal model分类
医药卫生引用本文复制引用
储德节,潘春峰,郭水根,王静,余竹元..肺炎衣原体诱发肺癌模型的建立[J].中国临床医学,2012,19(3):204-206,3.基金项目
上海市科委基金项目(编号:09411966300) （编号:09411966300）
