中国临床医学2012，Vol.19Issue(3)：209-212,4.
胃癌组织中microRNA-650的表达及其生物学作用和临床意义
Role of MicroRNA-650 in Gastric Cancer and Its Clinical Significance
摘要
Abstract
Objective:To explore the role of microRNA-650(miR-650) in the development and prognosis of gastric cancer and its clinical significance. Methods:Real-time quantitative reverse transcription-polymerase chain reaction was used to detect miR-650 expression levels in five gastric cancer cell lines and gastric cancer tissues. After the miR-650 mimic and inhibitor were transfected into gastric cancer cells,the cellular growth activity was estimated by CCK-8 kit; the apoptosis activity was tested by flow cytometry; the candidate targets of miR-650 were analysed using the LC-MS/MS-based techniques; western blot was used to test the expression levels of reglator of G-protein signaling 7(RGS7) which acted as a target of miR-650 in gastric cancer cells. Nude mouse xenograft model was used to explore the biological effect of miR-650 in gastric cancer cells and the curative effect of miR-650 inhibitor. Results: High levels of miR-650 have been detected in KATO III,NUGC4 and SGC-7901 gastric cancer cell lines,while NCI-N87 and MKN-45 gastric cancer cell lines only displayed low expression levels of miR-650. After transfection of LNA inhibitor,gastric cancer cell lines proliferation was significantly inhibited,while apoptosis was promoted. RGS7 was one of the functional downstream targets of miR-650,and the expression level of RGS7 was inversely correlated with the expression level of miR-650. MiR-650 inhibitor can suppress tumor growth. Conclusions: The up-regulation of miR-650 promotes proliferation,metastasis and inhibits apoptosis of gastric cancer cells,and possibly through suppressing the expression of RGS7.关键词
胃癌/凋亡/增殖/MicroRNA-650/G-蛋白信号调节因子7Key words
Gastric cancer/Apoptosis/Proliferation/MicroRNA-650/Regulator of G-protein signaling 7分类
医药卫生引用本文复制引用
霍守俊,薛锋,孔凡志,李芳,单远州,周联明,张学利..胃癌组织中microRNA-650的表达及其生物学作用和临床意义[J].中国临床医学,2012,19(3):209-212,4.基金项目
上海市自然科学基金资助(编号:08ZR1408900) （编号:08ZR1408900）
