中国临床药学杂志2012，Vol.21Issue(4)：218-221,4.
肠道转运Caco-2细胞单层模型的建立及验证评价
Development and validation of Caco-2 cell monolayer-model of intestinal transport
余自成 1高瑜 2田薇薇 1瞿江媛 1马明华 1陈红君 1王艳霞 3陈红专3
作者信息
- 1. 上海市杨浦区中心医院临床药学和药理学研究室,上海200090
- 2. 江苏徐州医学院
- 3. 上海交通大学医学院药理学教研室,上海200025
- 折叠
摘要
Abstract
AIM To develop a Caco-2 cell monolayer model for the investigation on drug intestinal transport. METHODS Caco-2 cell was seeded in Millicell plate inserts with 1×105 cells·mL-1 cell density and cultivated in standard procedures for 21 d. The value of transepithelial electrical resistance (TEER) of the monolayer was periodically monitored with a Millicell electrode resistance system (Millipore) for the evaluation of monolayer integrity. Lucifer yellow rejection was used to examine the permeability of paracellular transport, and propranolol transport was used to verify the permeability via passive diffusion mechanism. RESULTS After 21 d, TEER value reached (981 ± 123) Ω·cm2, the apparent permeability (Papp) of lucifer yellow and propranolol was 0.33×10-6 cm·s-1 and 16.7×10-6cm·s-1, respectively. These results showed that Caco-2 cell monolayer model developed in the present study was compact and complete with good permeability. CONCLUSION Caco-2 cell monolayer model is a valuable model system which can be used for the drug intestinal transport study.关键词
Caco-2细胞单层/肠道转运模型/跨上皮细胞电阻Key words
Caco-2 cell monolayer/ intestinal transport model/ transepithelial electrical resistance引用本文复制引用
余自成,高瑜,田薇薇,瞿江媛,马明华,陈红君,王艳霞,陈红专..肠道转运Caco-2细胞单层模型的建立及验证评价[J].中国临床药学杂志,2012,21(4):218-221,4.
