中国脑血管病杂志2012，Vol.9Issue(8)：418-422,427,6.DOI:10.3969/j.issn.1672-5921.2012.08.006
大黄酚对大鼠肾上腺髓质嗜铬细胞瘤分化株细胞缺氧损伤的保护作用
Protective effect of chrysophanol on hypoxic injury of rat adrenal medulla pheochromocytoma cells
摘要
Abstract
Objective To observe the protective effect of chrysophanol on hypoxic induced cell injury of rat adrenal medulla pheochromocytoma cells (PC12). Methods PC12 cells were cultured. A model of hypoxic caused PC12 cell injury was induced by using self-made airtight 3 gas box. The rats were divided into control, model and chrysophanol ( 1 μg/mL or 5 μg/mL) groups according to the different conditions. The proliferation activities of PC12 cells were determined by the tetrazolium salt (MTT) assay at 1, 2, 6 , 12 , and 24 hours before and after hypoxia treatment. The nuclear morphology of PC 12 cells was observed under the oil microscope. Superoxide dismutase (SOD) content in the supernatant in each group was detected. Immunohistochemical methods were used to detect the human homologous protein ( Bcl-2) expression of the C. Elegans anti-apoptotic gene in each group. Real-time quantitative PCR was used to detect the expressions of mitogen-activated protein kinase (p38 MAPK) and cysteine aspartic protease 3 (caspase-3) mRNA. Results ① The cell viability in the chrysophanol 1 μg/mL group detect by MTT assay at 12 hours after hypoxia treatment was higher than that in the model group; the cell viability of chry-sophanol 1 μg/mL group and chrysophanol 5 μg/mL group at 24 hours after hypoxia was higher than that in the model group. There were significant differences (P < 0.05). ②Beginning from hypoxia treatment for 1 hour, the SOD value in chrysophanol 1 μg/mL and 5|μg/mL groups was higher than that in the model group. There were significant differences (P<0.05). ③Beginning from 2 hours after hypoxia, the Bcl-2 positive rate in the chrysophanol 1 (μg/mL and 5 μg/mL groups was higher than that in the model group. ④Beginning from 2 hours after hypoxia treatment, the p38MAPK mRNA expression in the model group was higher than that in the chrysophanol 1 μg/mL and 5 μg/mL groups, there were significant differences ( P < 0. 05 ) . Beginning from 6 hour after hypoxia treatment, the caspase-3 mRNA expression in the model group was higher than that in the chrysophanol 1 μg/mL and chrysophanol 5 μg/mL groups. There were significant differences (P < 0. 05).⑤At 12 and 24 hours after hypoxia treatment, the nucleus shrunk, the cell morphology was unclear, and the formation of granular materials increased and became white in the model group; the nuclear morphology was clearer in the chrysophanol 1 μg/mL and 5 μg/mL groups, the formation of granular materials was rare. Conclusion Chrysophanol may reduce the hypoxia-induced PC12 cell injury and have a protective effect on PC12 cells.关键词
缺氧/肾上腺髓质/嗜铬细胞瘤/大黄酚/体外研究/大鼠/神经细胞保护药Key words
Hypoxia/Adrenal medulla /Pheochromocytoma/Chrysophanol/In vitro/Rats/Neuroprotective agents引用本文复制引用
荔志云,张明,季玮,周杰,田立桩,赵红斌,孙建军,李长栋..大黄酚对大鼠肾上腺髓质嗜铬细胞瘤分化株细胞缺氧损伤的保护作用[J].中国脑血管病杂志,2012,9(8):418-422,427,6.基金项目
甘肃省自然科学基金资助项目(096RJZA095) （096RJZA095）
