中国脑血管病杂志2012，Vol.9Issue(8)：423-427,5.DOI:10.3969/j.issn.1672-5921.2012.08.007
可卡因-苯丙胺调节转录肽对脑缺血-再灌注损伤小鼠脑保护机制的研究
Effect of cocaine-amphetamine-regulated transcript peptide on cerebral protective mechanisms in cerebral ischemia/reperfusion injury in mice
摘要
Abstract
Objective To investigate the effect of cocaine- and amphetamine-regulated transcript peptide (CART) on the infarct volume and the levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 3-nitrotyrosine (3-NT) in cerebral ischemia/reperfusion injury in mice. Methods A total of 264 healthy male Kunming mice (10 to 12 weeks of age) were included in the study. They were randomly divided into four groups: ischemia/reperfusion, CART, isotonic saline (n =72 in each group) , and sham operation ( n = 48) groups. A model of middle cerebral artery occlusion ( MCAO) for 2 hours and reperfusion for 12, 24, 48, and 72 hours was induced (n = 6 for each time point). Before reperfusion, CART55-102 (0.5μg, 12 nmol/L; repeated every 24 hours) was injected via tail veins in the CART group, and the same volume of isotonic saline were given in the isotonic saline group for control. A double antibody sandwich enzyme-linked immunosorbent assay was used to detect the levels of 8-OHdG and 3-NT in brain tissue. Results ① The infarct volume at each time point in the CART group was lower than that in the isotonic saline and the ischemia/reperfusion groups, and there were significant differences at 24, 48, and 72 hours ( P < 0. 01).②Compared to the sham operation group, the levels of 8-OHdG and 3-NT increased at each time point after reperfusion in the ischemia/reperfusion, CART and isotonic saline groups. 8-OHdG and 3-NT reached the peaks at 12 and 48 hours respectively.③Compared to the ischemia/reperfusion and isotonic saline groups, the levels of 8-OHdG and 3-NT in brain tissue were down-regulated at each time point after reperfusion in the CART group. There was only significant difference in 8-OHdG at 12 hours (P < 0. 05 ) and there were significant difference in 3-NT at 24 , 48 , and 72 hours ( P < 0. 05 ). Conclusion The protective effect of CART on cerebral ischemia/reperfusion injury in mice may be associated with the downregulation of 8-OHdG and 3-NT levels and the inhibition of oxidative stress.关键词
脑梗死/再灌注损伤/小鼠/可卡因-苯丙胺调节转录肽/8-羟基-2'-脱氧鸟苷/3-硝基酪氨酸Key words
Brain infarction/Reperfusion injury/Mice/Cocain- and amphetamine-regulated transcript peptides/S-hydroxy-2-deoxyguanosine/3-nitrtyrosine引用本文复制引用
沙杜鹃,韩勇,顾双双,王璐娜,朱震寒,李瑾,李启明,张均..可卡因-苯丙胺调节转录肽对脑缺血-再灌注损伤小鼠脑保护机制的研究[J].中国脑血管病杂志,2012,9(8):423-427,5.基金项目
南京市医学科技发展项目(YKK10069) （YKK10069）
