中国农业科学2012，Vol.45Issue(7)：1387-1391,5.DOI:10.3864/j.issn.0578-1752.2012.07.017
中国荷斯坦奶牛IL-8基因SNP-180A/G显著影响体细胞评分
IL-8 Gene SNP-180A/G Affects Significantly Somatic Cell Score in Chinese Holstein Cow
摘要
Abstract
[Objective] Bovine interleukin-8 (IL-8) is one of the major factors recruiting neutrophils during cow mastitis and causing rapid increase of somatic cell counts (SCC). Knowledge of IL-8 polymorphisms would be helpful to genetic selection of low SCC. [Method] The SNPs in the 5' upstream of bovine IL-8 gene were investigated in 610 Chinese Holstein cows from 30 bull families in a dairy farm in Shanghai using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique, and milking traits and somatic cell score (SCS) were measured and their relationship was analyzed using a mixed animal model. [Result] The results showed that one SNP, A(-180)G, was detected, determining three genotypes, GG, GA and AA with frequencies of 0.41, 0.43 and 0.16, respectively. The SNP showed significant effect on SCS, with GG having a significantly lower SCS than GA or AA (P<0.01), while this SNP had no effect on test-day milk yield, test-day milk fat content, test-day milk protein content, and 305 d corrected milk yield. [Conclusion]The findings demonstrated that the SNP -180A/G exerted significant effect on SCS.关键词
中国荷斯坦奶牛/IL-8基因/SCS/SNP/泌乳性状Key words
Chinese Holstein cow/IL-8 gene/SCS/SNP/milking traits引用本文复制引用
冀德君,陈仁金,杨章平,朱国强,毛永江,陈莹,李锐..中国荷斯坦奶牛IL-8基因SNP-180A/G显著影响体细胞评分[J].中国农业科学,2012,45(7):1387-1391,5.基金项目
国家“863”计划项目(2008AA10Z144、2008AA101010)、江苏省高校自然科学基金重大项目(09KJA230002)、扬州大学高层次人才基金(2009) （2008AA10Z144、2008AA101010）
