中国农业科学2012，Vol.45Issue(13)：2668-2675,8.DOI:10.3864/j.issn.0578-1752.2012.13.011
GA含量与其合成酶基因在‘长富2号’苹果及其短枝型芽变品种之间的比较分析
Comparison of Gibberellin Acid Content and the Genes Relatived to GA Biosynthesis Between ‘Changfu 2’ Apple (Malus domestica Borkh.) and Its Spur Sport
摘要
Abstract
[Objective] The relationship between intemode length of shoots, endogenous hormones gibberellic acid (GA) and the structure and expression of genes related to GA synthesis in apple (Mains domesiica Borkh.) is a basis for further exploration of the spur type development mechanism and breeding of cultivars of spur type apple. [ Method ] The shoots and leaves of 'Changfu 2' and its spur sport 'Longfuduanzhi' were used as experimental materials for measuring the content of GA and cloning the genes which involved in the GA synthesis pathway at the four time points days after flowering and researching the relationship between GA and the intemode length of shoots at shoot development stage. [ Result ] The GA content was significantly different between the spur type apple and non-spur type apple on 80th day after flowering. The GA content was lower in spur type sport compared with in non-spur type apple varieties on 80th day after flowering. The sequences analysis showed that the cDNA sequences of GA20ox and KO, key genes involved in GA synthesis, were the same between 'Longfuduanzhi' and 'Changfu 2'. The mutation, insertion and deletion were not appeared in GA20ox and KO. The real-time quantitative PCR analysis showed that the relative expression of GA20ox and KO were significantly different between 'Longfuduanzhi' and 'Changfu 2' on 20th and 80th day after flowering. The relative expression of GA20ox and KO was lower in spur type apple compared with non-spur type apple on 20th and 80th day after flowering. [ Conclusion ] The research results showed that the difference of GA content and key genes expression of GA synthesis pathway were related to shoot internode length of spur sport apple. The lower GA content and genes involved in GA synthesis pathway were down-regulated expression which inhibited the normal development of spur sport apple shoots. This study has provided basic information for further exploration of the spur type development mechanism.关键词
短枝型苹果/赤霉素/实时荧光定量PCRKey words
spur type apple/ gibberellin acid/ real-time PCR引用本文复制引用
宋杨,张艳敏,刘金,王传增,刘美艳,冯守千,陈学森..GA含量与其合成酶基因在‘长富2号’苹果及其短枝型芽变品种之间的比较分析[J].中国农业科学,2012,45(13):2668-2675,8.基金项目
国家自然科学基金项目(31171932)、国家重点基础研究发展计划项目(2011CB100606) （31171932）
