中国农业科学2012，Vol.45Issue(13)：2728-2736,9.DOI:10.3864/j.issn.0578-1752.2012.13.017
FSHR基因第10外显子多态性及其与小梅山猪产仔数的相关性
Polymorphism of Exon10 of FSHR Gene and Its Relationship with Litter Size in Xiaomeishan Pigs
摘要
Abstract
[Objective] The effect of polymorphism of exon10 of FSHR gene on litter size in Xiaomeishan pigs was studied. [ Method] The polymorphism of exon10 of FSHR gene was investigated by PCR-SSCP and direct sequencing methods, and the effect of exonlO of FSHR gene on litter size traits in Xiaomeishan pigs was analyzed using the least square analysis. Simultaneously the variance components were analyzed and selective reaction was predicted. [Result] There was one novel polymorphic locus dected by primers P1 and P3, respectively, and the former made amino acid change, among Xiaomeishan, Fengjing and Large White pigs. One polymorphic locus detected by primer P6 was only in Large White pigs. Allele A at locus P1 and allele D at P3 locus were the dominant alleles in Xiaomeishan pigs. AA genotypic sow of Xiaomeishan pigs produced 1.95 TNB (total number born) and 1.66 NBA (number born alive) more than BB genotypic sow at locus P1 after the second parity, respectively (P<0.01). And DD genotypic sow of Xiaomeishan pigs produced more TNB and NBA than CC genotypic sow at locus P3 after the second parity and in all parities, respectively (P<0.01). The heredity of TNB and NBA in Xiaomeishan pigs was mostly influenced by the genetic additive effect. [Conclusion] Allele A at locus P1 and allele D at locus P3 in exon10 of FSHR gene had significant effect on litter sizes in Xiaomeishan pigs.关键词
FSHR基因/多态性/小梅山猪/PCR-SSCPKey words
FSHR gene/ polymorphism/ Xiaomeishan pigs/ PCR-SSCP引用本文复制引用
吴井生,王金玉..FSHR基因第10外显子多态性及其与小梅山猪产仔数的相关性[J].中国农业科学,2012,45(13):2728-2736,9.基金项目
江苏省农业委员会农业三项工程项目(sx＜2009＞25,sx＜2010＞244)、江苏省畜禽技术改进项目(2009＜46＞) （sx＜2009＞25,sx＜2010＞244）
