中国农业科学2012，Vol.45Issue(13)：2737-2742,6.DOI:10.3864/j.issn.0578-1752.2012.13.018
hLF转基因羊繁殖、遗传与表达稳定性
Breeding and Expression Stability of Human Lactoferrin Transgenic Goat
摘要
Abstract
[Objective] This study was conducted to analyze the effects of transgenosis on the reproduction of human lactoferrin (hLF) transgenic goat, and the genetic and expression stability of exogenous gene in each generation of transgenic animals. [Method] Family line was established with self-developed hLF transgenic goats (G0♂), F1 generation was composed by integrated offspring individuals of G0♂ mated with ordinary dairy goat, F2 was generated by F1 males mated with F1, F2 or ordinary females, and F3 was generated by F2 males mated with F2 females. PCR and ELISA were implied to identify the integration and expression of exogenous gene in offsprings. Reproductive data such as quantity of offspring with different mating patterns, rate of transgenic integrated individuals, conception rates, abnormal rates of lamb in each generation were counted statistically. [Result] After several reproductive cycles, no abnormalities of conception rates and lambing rates were observed. The results showed that, there were 19 hLF transgenic individuals in F, generation, with integration rate of 24.7% (19/77), 86 individuals in F2 generation, in which 62 were generated by F1♂×N♀, 16 were obtained by F1♂×F1♀, and 8 were obtained by F1♂×F2♀, with the rate of 33.2% (62/187), 76.2% (16/21) and 34.8% (8/23), respectively, 9 hLF transgenic individuals in F3 generation and the integration rate of F1 was 52.9% (9/17). ELISA was conducted to measure the expression of hLF in F1, F2 and F3 in 20 weeks, and the results showed that hLF expressed stably in each transgenic generation and the average expression level in milk were 351.75, 340.00 and 326.25 μg·mL-1, respectively. [Conclusion] The results suggested that, there is no significant effect of transgenosis on reproductive performance of hLF transgenic goat, and exogenous hLF gene could inherit and stably express in each generation of goats.关键词
人乳铁蛋白/转基因山羊/繁殖/遗传/表达稳定性Key words
human lactoferrin/ transgenic goat/ breeding/ hereditary/ expression stability引用本文复制引用
乔娜,徐旭俊,刘思国,王学斌,俞慧清,童永星,陈建泉,成国祥..hLF转基因羊繁殖、遗传与表达稳定性[J].中国农业科学,2012,45(13):2737-2742,6.基金项目
国家转基因生物新品种培育科技重大专项(2009ZX08011-032B)、上海市科技兴农攻关项目(沪农科攻字(2011)第4-10号)、上海市科学技术委员会项目(10DZ2251700) （2009ZX08011-032B）
