内蒙古绒山羊CRABP Ⅰ基因的克隆及表达OA北大核心CSCDCSTPCD
Cloning and Expression of Cellular Retinoic Acid Binding Protein Ⅰ Gene in Inner Mongolian Cashmere Goats
[目的]克隆内蒙古绒山羊视黄酸结合蛋白Ⅰ (cellular retinoic acid binding proteinⅠ,CRABP Ⅰ)基因的cDNA,进行蛋白结构的预测和表达分析,为绒山羊毛和绒形成的分子机理奠定基础.[方法]利用RT-PCR 方法克隆内蒙古绒山羊CRABP Ⅰ基因序列,利用生物信息学方法预测其蛋白结构,采用实时荧光定量PCR探讨该基因在绒山羊4个胎儿日龄皮肤中的表达.[结果]内蒙古绒山羊CRABP Ⅰ基因cDNA长679 …查看全部>>
[Objective] The cDNA sequence of cellular retinoic acid binding protein I (CRABP I) gene was cloned in Inner Mongolian cashmere goats, and the protein structure gene and expression were also analyzed. All these would establish a foundation for molecular mechanism of follicle and cashmere formation. [Method] The cDNA sequence of CRABP I gene was cloned by RT-PCR in Inner Mongolian cashmere goats. The protein structure was predicted through bioinformatics appr…查看全部>>
李华;苏立宁;刘东军;李雪峰;旭日干
内蒙古大学生命科学院/哺乳动物生殖生物学及生物技术教育部重点实验室,呼和浩特010021佛山科学技术学院,广东佛山528231佛山科学技术学院,广东佛山528231华南师范大学生命科学学院,广州510000河北北方学院,河北张家口075000
绒山羊CRABP Ⅰ基因克隆基因表达生物信息学
cashmere goats CRABP I gene clone gene expression bioinformatics
《中国农业科学》 2012 (13)
2743-2750,8
国家转基因项目(2008zx08008-002)
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