中国农业科学2012，Vol.45Issue(13)：2743-2750,8.DOI:10.3864/j.issn.0578-1752.2012.13.019
内蒙古绒山羊CRABP Ⅰ基因的克隆及表达
Cloning and Expression of Cellular Retinoic Acid Binding Protein Ⅰ Gene in Inner Mongolian Cashmere Goats
摘要
Abstract
[Objective] The cDNA sequence of cellular retinoic acid binding protein I (CRABP I) gene was cloned in Inner Mongolian cashmere goats, and the protein structure gene and expression were also analyzed. All these would establish a foundation for molecular mechanism of follicle and cashmere formation. [Method] The cDNA sequence of CRABP I gene was cloned by RT-PCR in Inner Mongolian cashmere goats. The protein structure was predicted through bioinformatics approach, the mRNA expression of the gene at four embryo ages in skin of cashmere goat were detected through real time PCR. [Result] The length cDNA is 679 bp (JN936490) , its open reading frame (ORF) is 414 bp, which shares high similarity with other species. CRABP I protein has no obvious signal peptide, transmembrane segments, N-glycosylation sites and O-glycosylation sites. The secondary structure of CRABP I protein consisted of mainly β sheets, a helixes and loops, also few turn and coil. CRABP 1 gene in cashmere goats was highly expressed on 90 d compared with on 100 d, 120 d and 130 d (P<0.05). [Conclusion] The open reading frame (ORF) of CRABP I gene is conserved among different species, but it has characteristics at 33 and 123 amino sites in cashmere goats. The level of mRNA expression was the highest on 90 d at four embryo stages, the polymorphism of this gene in different breeds and its regulation mechanism to sebaceous gland of hair follicle needs to be studied further.关键词
绒山羊/CRABP Ⅰ基因/克隆/基因表达/生物信息学Key words
cashmere goats/ CRABP I gene/ clone/ gene expression/ bioinformatics引用本文复制引用
李华,苏立宁,刘东军,李雪峰,旭日干..内蒙古绒山羊CRABP Ⅰ基因的克隆及表达[J].中国农业科学,2012,45(13):2743-2750,8.基金项目
国家转基因项目(2008zx08008-002) （2008zx08008-002）
