中国农业科学2012，Vol.45Issue(13)：2751-2757,7.DOI:10.3864/j.issn.0578-1752.2012.13.020
应用 DNA 环介导恒温扩增技术快速检测空肠弯曲菌
Rapid Detection of Campylobacter jejuni Using Loop-Mediated Isothermal Amplification Assay
摘要
Abstract
[ Objective ] Campylobacterjejuni is a major zoonotic pathogen, mainly initiates its transmission with contaminated water, milk products and raw poultry. Thus, the development of a rapid and accurate diagnostic method is an important measure to prevent and control C.jejuni. [Method] Loop-mediated isothermal amplification (LAMP) assay was introduced to develop a rapid method for the detection of C.jejuni using the ORF-C sequence of C.jejuni as the target gene. [ Result] Twenty-one bacterial strains were used to evaluate the specificity of the LAMP method. The result showed that only C.jejuni was positive and the other strains were negative in LAMP, indicating that the LAMP method was highly specific to the target pathogen with the sensitivity of 5CFU per test tube for C.jejvni cultivated and 9 CFU per test tube for its contaminated materials. [ Conclusion] The LAMP method developed in this work is a sensitive, rapid and simple tool for the detection of C.jejuni.关键词
空肠弯曲菌/环介导恒温扩增/快速检测Key words
Campylobacterjejuni/ LAMP/ rapid detection引用本文复制引用
徐义刚,李丹丹,田长永,刘忠梅,李苏龙,张国财..应用 DNA 环介导恒温扩增技术快速检测空肠弯曲菌[J].中国农业科学,2012,45(13):2751-2757,7.基金项目
国家质检总局科技计划项目(20081K162)、黑龙江省博士后基金(LBH-Z09) （20081K162）
