中国农业科学2012，Vol.45Issue(13)：2771-2778,8.DOI:10.3864/j.issn.0578-1752.2012.13.023
新疆红肉苹果果皮果肉呈色差异机理
The Mechanism of Red Coloring Difference Between Skin and Cortex in Malus sieversii f.neidzwetzkyana (Dieck) Langenf.
摘要
Abstract
[Objective] The aim of this study is to explore the red development mechanism by monitoring anthocyanin composition and content, transcnptional profile of anthocyanin related genes in Mains sieversiif. neidzwetzkyana (Dieck) Langenf. [Method] M. sieversiif. neidzwetzkyana (Dieck) Langenf was used as material to study anthocyanin composition of skin and cortex by HPLC, and to study the transcnptional profile of anthocyanin related genes by qPCR at four developmental stages. Anthocyanin contents in skin and cortex were determined at the four sampling times accordingly. [ Result] Cyanidin-3-galactoside was the main anthocyanin composition of skin and cortex, but anthocyanin content had different change trends at different developmental stages. The anthocyanin content of skin showed a declined pattern, while the anthocyanin content of cortex displayed an opposite tendency. Expression of the anthocyanin biosynthesis genes was correlated with anthocyanin content: expression of MsMYB10 in skin and cortex was very high, while expression of MsMYB10 showed an increasing pattern in skin and a ' high-low-high-low' change trend in cortex during fruit development. [Conclusion]The red coloring difference between skin and cortex of 'Xiahongrou' mainly relate to the anthocyanin content and the different expressions of anthocyanin gene. These results have provided a foundation for further exploring the red coloring mechanism and breeding cultivars of red-flesh apple.关键词
新疆红肉苹果/花青苷/MsMYB10转录因子/基因表达Key words
Mains sieversii f. neidzwetzkyana/ anthocyanins/ MsMYB10/ gene expression引用本文复制引用
王延玲,张艳敏,冯守千,宋杨,徐玉亭,张友朋,陈学森..新疆红肉苹果果皮果肉呈色差异机理[J].中国农业科学,2012,45(13):2771-2778,8.基金项目
国家重点基础研究发展计划课题项目(2011CB100606)、国家自然科学基金项目(31171932)、山东省农业良种工程项目 （2011CB100606）
