绵羊UCP1基因碱基突变与其蛋白结构和功能OA北大核心CSCDCSTPCD
The Base Mutation of Ovine UCP1 Gene and Its Relationship with Protein Structure and Function
[目的]检测两个绵羊群体中解偶联蛋白1(uncoupling protein 1,UCP1)基因的单核苷酸多态性(single nucleotide polymorphism,SNP),预测和分析碱基改变对蛋白结构和功能的影响.[方法]利用PCR-SSCP结合测序的方法检测UCP1基因编码区的SNPs,用生物信息学方法分析UCP1蛋白的理化性质和结构.[结果]UCP1基因编码区存在4个SNPs,其中c.214G＞A (Va172Met)和c.27…查看全部>>
[Objective] The objectives of this study are to detect the single nucleotide polymorphism (SNP) of UCP1 gene in two fat-tailed sheep breeds, and to predict the protein structure and function caused by the mutations. [Method] PCR-SSCP combined with sequencing were used to detect the SNPs in coding region of UCP1 gene, and bioinformatics approach was used to predict the physicochemical properties and structures of UCP1 protein. [Result] Four SNPs existed in th…查看全部>>
袁亚男;刘文忠;刘建华;乔利英;武建亮
山西农业大学动物科技学院,山西太谷030801山西农业大学动物科技学院,山西太谷030801山西农业大学动物科技学院,山西太谷030801山西农业大学动物科技学院,山西太谷030801山西农业大学动物科技学院,山西太谷030801
绵羊解偶联蛋白1(UCP1)单核苷酸多态性(SNP)生物信息学蛋白结构
sheep uncoupling protein 1 (UCP1) single nucleotide polymorphism (SNP) bioinformatics protein structure
《中国农业科学》 2012 (14)
不同脂尾型绵羊脂肪代谢相关基因遗传机制的研究
2973-2980,8
国家自然科学基金项目(30972084)、山西省研究生优秀创新项目(20103042)
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