中国农业科学2012，Vol.45Issue(14)：2973-2980,8.DOI:10.3864/j.issn.0578-1752.2012.14.021
绵羊UCP1基因碱基突变与其蛋白结构和功能
The Base Mutation of Ovine UCP1 Gene and Its Relationship with Protein Structure and Function
摘要
Abstract
[Objective] The objectives of this study are to detect the single nucleotide polymorphism (SNP) of UCP1 gene in two fat-tailed sheep breeds, and to predict the protein structure and function caused by the mutations. [Method] PCR-SSCP combined with sequencing were used to detect the SNPs in coding region of UCP1 gene, and bioinformatics approach was used to predict the physicochemical properties and structures of UCP1 protein. [Result] Four SNPs existed in the coding region, with c.214G>A (Val72Met) and C.273C>T located on exon 2, C.624C>T and and C.757C>A (Ala253Thr) on exon 5. Further analysis indicated that the two mutations which caused amino acid substitution had little influence on the protein expression. This was because of the two mutations merely resulted in a slight change of the physicochemical properties and transcription factor binding site, but did not lead to the change of its spatial configuration. [Conclusion] Comparison of UCP1 protein structures between two exonic mutants, which associated respectively with human obesity and diabetes, and the normal structure suggested that the changes in UCP1 protein structure could influence the protein function.关键词
绵羊/解偶联蛋白1(UCP1)/单核苷酸多态性(SNP)/生物信息学/蛋白结构Key words
sheep/ uncoupling protein 1 (UCP1)/ single nucleotide polymorphism (SNP)/ bioinformatics/ protein structure引用本文复制引用
袁亚男,刘文忠,刘建华,乔利英,武建亮..绵羊UCP1基因碱基突变与其蛋白结构和功能[J].中国农业科学,2012,45(14):2973-2980,8.基金项目
国家自然科学基金项目(30972084)、山西省研究生优秀创新项目(20103042) （30972084）
