中国普通外科杂志2012，Vol.21Issue(7)：821-824,4.
骨髓c-Kit+Lin-细胞移植改善肝纤维化的实验研究
Bone marrow c-Kit+Lin- cells transplantation for hepatic fibrosis:an experimental study
摘要
Abstract
To investigate the therapeutic effect of in vitro amplified bone marrow c-Kit+Lin (CD117) cells transplantation on hepatic fibrosis in mice.Methods: The bone marrow c-kit+Lin cells of mice were isolated and purified by using magnetic-activated cell sorting (MACS), and then the cells were cultured and expanded in the serum-free medium supplemented with hepatocyte growth factor (HGF), stem cell factor (SCF), FLt-3 ligand (FL), thrombopoietin (TPO) and leukemia inhibitory factor (LIF). The models of alcohol induced hepatic fibrosis were created in mice of the same strain, and the model mice were treated with CD117 cells (transplantation group) or buffer solution of the same volume (model group) by tail vein injection respectively, and the normal mice were used as controwere significantly decreased (both P<0.0l) while their vimentin levels were significantly increased (both P<0.01); Transwell chamber results indicated that the migratory and invasive abilities of both types of HCC cells were significantly enhanced under hypoxic system (all P<0.01); and all the above changes were more evident in SMMC-7721 cells than those in HepG2 cells (all P<0.0l).关键词
肝硬化/干细胞移植/CD117/疾病模型,动物Key words
Hypoxia can induce EMT process in HCC cells and enhance their invasive and migratory ability. Liver Neoplasms/ Cell Hypoxia/ Epithelial-Mesenchymal Transition/ Neoplasm Invasiveness分类
医药卫生引用本文复制引用
张守华,肖菊花,邓庆强,徐维,胡瞒鑫,谌卫龙,陶强..骨髓c-Kit+Lin-细胞移植改善肝纤维化的实验研究[J].中国普通外科杂志,2012,21(7):821-824,4.基金项目
江西省卫生厅科技计划资助项目(20113098) （20113098）
江西省科技支撑计划资助项目(2010BSA11200). （2010BSA11200）
