中国人兽共患病学报2012，Vol.28Issue(6)：539-543,5.DOI:10.3969/cjz.j.issn.1002-2694.2012.06.005
CVB3损伤HeLa细胞高尔基体结构的机制研究
Mechanism for structure damage of Golgi apparatus in HeLa cells by CVB3
摘要
Abstract
We investigated the damage mechanism of Coxsackievirus B3 (CVB3) in Golgi apparatus and discussed the pathogenic mechanism of its infection. After infecting HeLa cells with CVB3 (MOI = 5), we observed the variation of Golgi ribbon by detecting the locations of viral protein VP1, cellular proteins Golgi matrix protein 130 (GM130), and Golgi reassembly stacking protein 65 (GRASP65) in HeLa cells at different time points post-infection with double-label immunofluorescence. Total cellular proteins were collected for quantitative analysis by Bradford Protein Assay at different post-infection times. And then the expressions of viral protein VP1, cellular proteins GM130, and GRASP65 in HeLa cells were analyzed by Western blot. Results showed that there were banding-like immunofluorescence pattern for GM130 in normal HeLa cells, and the structure of Golgi apparatus was completes while in the CVB3 infected HeLa cells, the immunofluorescence pattern of GM130 became scattered point-like and the structure of Golgi apparatus was damaged in the infected cells after 3 h post-infection. Meanwhile, the expression of cellular proteins GM130 and GRASP65 decreased gradually after the infection and the expression of viral protein VP1 maintained at a high level after 6 h post-infection. Its suggested that CVB3 infection down-regulates the expressions of GM130 and GRASP65 in HeLa cells and induces diffusion in immunofluorescence patterns of GM130 and GRASP65 as well as disruption in Golgi ribbon, which might be a possible pathogenic mechanism for Golgi apparatus structure damage in CVB3 infected host cells.关键词
CVB3/高尔基体/GM130/GRASP65/VP1Key words
CVB3/ Golgi apparatus/ GM130/ GRASP65/ VP1分类
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金桂林,莫冰,刘发娣,徐秋芳,刘昕,应颖,赵颖洁,罗军,黄孝天..CVB3损伤HeLa细胞高尔基体结构的机制研究[J].中国人兽共患病学报,2012,28(6):539-543,5.基金项目
国家自然科学基金(30660010、81160196),江西省自然科学基金(0540043),江西省教育厅科技计划项目(2006323),江西省科技厅2008年科技支撑计划项目“柯萨奇病毒VP1对高尔基体结构影响研究” （30660010、81160196）
