中国人兽共患病学报2012，Vol.28Issue(6)：544-548,560,6.DOI:10.3969/cjz.j.issn.1002-2694.2012.06.006
非O157产志贺毒素大肠杆菌分离株的多位点序列分型研究
Multilocus sequence typing of non-O157 Shiga toxin-producing Escherichia coli isolates
摘要
Abstract
To investigate the molecular epidemiologic characteristics of non-0157 Shiga toxin-producing Escherichia coli isolates collected from some locations in China over a period of time, a multilocus sequence typing (MLST) scheme provided by Escherichia coli MLST database was performed. Seven housekeeping genes were amplified by PCR and then sequenced. The sequence types (STs) were determined by sequence comparison. The MEGA and eBURST were used for phylogenetic analysis, MLST analysis showed that there were 13 STs identified in 29 isolates, including 3 new STs named ST2460, ST2467 and ST2468. The predominant sequence type was ST155, which consisted of 10 isolates (34. 48%), following by ST906 (17. 24%), ST446 (10. 44%) and ST33 (6. 90%). The other 9 STs consisted of only one isolate. It could be concluded that there exists molecular diversity among the non-()157 Shiga toxin-producing Escherichia coli isolates, which showed genetic relationship with the popular strains worldwide. The detection and surveillance of this kind of isolates is of great significance to public health.关键词
非O157产志贺毒素大肠杆菌/多位点序列分型/序列型Key words
non-0157 Shiga toxin-producing Escherichia coli/ multilocus sequence typing/ sequence type分类
医药卫生引用本文复制引用
白向宁,赵爱兰,夏胜利,熊衍文,徐建国..非O157产志贺毒素大肠杆菌分离株的多位点序列分型研究[J].中国人兽共患病学报,2012,28(6):544-548,560,6.基金项目
国家“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项(2008ZX10004-009)资助,赵爱兰与白向宁同等贡献 （2008ZX10004-009）
