中国人兽共患病学报2012，Vol.28Issue(6)：574-578,591,6.DOI:10.3969/cjz.j.issn.1002-2694.2012.06.012
广州管圆线虫感染小鼠脾脏T淋巴细胞亚群和细胞因子的变化
Alteration of T-cell subtype and cytokine in spleen of mice infected with Angiostrongylus cantonensis
摘要
Abstract
To investigate the changes of T-cell subtypes and cytokine level in spleen of mice infected with Angiostrongylus cantonensis, BAI.B/c mice were infected with third-stage larva of Angiostrongylus cantonensis from Achatina fulica. The splenocytes of mice were collected and cultured on the 19th and 25th day post-infection. The subtypes of T-cells were detected by Flow Cytometry with fluorescence staining and the cytokines in cultured supernatants of splenocytes were assayed by the method of EI-ISA. Results showed that the percentages of CD4+ and CD4+ IL-4+ T cells in splenocytes of infected mice were much higher than those in normal control mice, however, the percentages of CD4+ IL-17 +and CD4+ IFN-γ+ T cell were much lower. In comparison with normal control group, the concentration of IL-4 in cultured supernatants of splenocytes in infected groups increased significantly, while that of IL-17 decreased significantly. In addition, the factors, such as the number of larva infected and days after infection, could influence the T-cell subtypes and their cytokine levels in spleen. It is evident that the CD4+T lymphocytes of spleen in the mice infected with Angiostrongylus cantonensis proliferate markedly and polarize to Th2 phenotypes.关键词
广州管圆线虫/T淋巴细胞亚群/细胞因子Key words
Angiostrongylus cantonensis/ T lymphocyte subtypes cytokines分类
医药卫生引用本文复制引用
罗晓东,沈二霞,陈代雄,丁雪,李华..广州管圆线虫感染小鼠脾脏T淋巴细胞亚群和细胞因子的变化[J].中国人兽共患病学报,2012,28(6):574-578,591,6.基金项目
国家自然科学基金项目(30972577) （30972577）
广东省自然科学基金项目(s2011040003741和9151051501000073) （s2011040003741和9151051501000073）
