中国人兽共患病学报2012，Vol.28Issue(6)：583-586,4.DOI:10.3969/cjz.j.issn.1002-2694.2012.06.014
福建省2005-2010年志贺菌分离株的毒力基因分析
Prevalence of virulence genes in isolates of Shigella species from Fujian, 2005-2010
摘要
Abstract
Investigation on the prevalence of virulence genes among Shigella spp. will reveal current circulating mode of different groups and serotypes. In this study, virulence genes, i. e. , ipaH, setKsetlA and setlB), sen and ial, in 104 Shigella isolates from Fujian, 2005-2010, were detected by PCR. The positive rates of these genes and their distribution modes were analyzed. It was revealed that positive rates for ipaH, set\, sen, and ial genes were 100% , 36. 54% , 66. 35% , and 64. 42% , respectively. Notably, the positive rates of sell were 85% and 6. 25% for S. flexneri and S. sonnei, respectively. Based on their virulence gene distribution mode, the 104 Shigella strains were divided into 8 groups. Group Ⅳ was the dominant mode (67. 5%)for S. flexneri, while GroupⅥ was the dominant mode(39. 06%)for S. sonnei. Meanwhile, the distribution modes of Group Ⅰ, Ⅶ and Ⅴ were relatively common in S. sonnei. These results indicated that ipaH could be used as a target gene for identification of Shigella spp. , and that sell was the mainly harbored virulence gene in S. flexneri. The observation that Group Ⅳ was the unique dominant circulating mode in S. flexneri and there were more relatively clustered modes in S. sonnei, suggested that the evolution process of Shigella spp. involved complicated differentiation, and that insertion and deletion of virulence genes was associated with variation and differentiation of Shigella spp.关键词
志贺菌属/毒力基因/流行模式Key words
Shigella species/ virulence gene/prevalence mode分类
医药卫生引用本文复制引用
陈爱平,李海丹,熊美琴,许英英,杨劲松,罗朝晨,郑金凤,严延生..福建省2005-2010年志贺菌分离株的毒力基因分析[J].中国人兽共患病学报,2012,28(6):583-586,4.基金项目
国家科技重大专项(No.2009ZX10004-210) （No.2009ZX10004-210）
