中国人兽共患病学报2012，Vol.28Issue(6)：607-611,5.DOI:10.3969/cjz.j.issn.1002-2694.2012.06.019
宫颈癌组织中HPV16型E6/E7序列突变分析
Mutations in the E6/E7 genes of human papillomavirus type 16 from cervical cancer tissue
摘要
Abstract
To investigate mutations in E6/E7 genes of human papillomavirus type 16 (HPV16) in patients with cervical cancer in Quanzhou area and explore the potential association between the mutations and cervical cancer, 35 cervical cancer tissue with HPV 16 positive were collected in this study. DNA samples were amplified by polymerase chain reation (PCR), then the products were directly sequenced and the results were compared with the prototype sequence. It was found that the prevalences of HPV 16 E6 and E7 variants were 91. 4% and 89. 2% respectively. Ten mis-sense variantions and 2 silent variantions were identified in E6. The hot spot of E6 nucleotide mutation was D25E, with a frequency of 77. 1%. A total of 5 mutation spots was found in E7, including 2 mis-sense and 3 silent variations. Both N29S and T846C were the most common mutations, with the same ratio of 75. 0%. It is suggested that the mutation of D25E, N29S and T846C are likely to be associated with ontogenesis of cervical cancer. This founding might provide valuable information for HPV vaccine development in China.关键词
人乳头状瘤病毒/宫颈癌/16型/E6基因/E7基因Key words
human papillomavirus/ type 16/ cervical cancers E6 genes E7 gene分类
医药卫生引用本文复制引用
张志珊,庄建良,李爱禄,蒋燕成..宫颈癌组织中HPV16型E6/E7序列突变分析[J].中国人兽共患病学报,2012,28(6):607-611,5.基金项目
福建省青年人才项目基金(2008F3123) （2008F3123）
福建医科大学科技发展专项基金(FZS08004)联合资助 （FZS08004）
