中国人兽共患病学报2012，Vol.28Issue(7)：653-658,663,7.DOI:10.3969/cjz.j.issn.1002-2694.2012.07.002
微小隐孢子虫含EF-hand结构域蛋白家族的生物信息学研究
Bioinformatics analysis on EF-hand domain contained protein family of Cryptosporidium parvum
摘要
Abstract
The propose of this study is to conduct bioinformatics research about the EF-hand domain contained protein family of Cry ptosporidium parvum , and then predict the relationship between the EF-hand domains and the Ca2+ signal which may provide a new direction for the diagnosis and treatment of the cryptosporidiosis. Firstly, based on the data collection, we acquired EF-hand domain contained protein family's sequences. Then the CDD (Conserved Domain Database) domain search and alignment analysis were conducted through the NCBI for every protein to ensure that all of them were EF-hand domain contained proteins. Lastly, we processed the domain analysis and functional prediction. Accordingly, we obtained 18 EF-hand domain contained proteins of C parvum Ⅰowa Ⅱ, and found that there were significant differences among their amino acid sequence length, molecular weight, isoelectric point, etc. From domain analysis, these 18 proteins could be classified into 4 classes, which were 1 EF-hand domain contained proteins, 2 EF-hand domains contained proteins, EF-hand and STKc-AGC domains contained proteins, and EF-hand and PKC domains contained proteins, respectively. Then we found that this type of protein was closely related to the calcium signals, and the C. parvum's host cell invasion ability had an important relationship with the intracellular Ca2+ levels. Hence, the EF-hand proteins family might become a new breakthrough for the treatment of cryptosporidiosis.关键词
微小隐孢子虫/EF-hand结构域/Ca2+/生物信息学分析/功能预测Key words
Cry ptosporidium parvum/ EF-hand domain/ Ca2+/ bioinformatics! functional prediction分类
医药卫生引用本文复制引用
郭志云,杨光,荆春霞,付芹芹,孙小会,王穗湘,李月琴,周天鸿..微小隐孢子虫含EF-hand结构域蛋白家族的生物信息学研究[J].中国人兽共患病学报,2012,28(7):653-658,663,7.基金项目
国家自然科学基金(30901249,81101267),广东省自然科学基金(I0151063201000036,S2011010002526)和广东省医学科研基金(B2007097)联合资助 （30901249,81101267）
