中国人兽共患病学报2012，Vol.28Issue(7)：739-744,6.DOI:10.3969/cjz.j.issn.1002-2694.2012.07.019
环介导等温扩增技术在致病菌检测中的应用
Application of LAMP in detection of pathogenic microbe
贺尔娜 1蔡挺 2张顺2
作者信息
- 1. 宁波大学医学院,宁波315211
- 2. 宁波大学附属第二医院,宁波315010
- 折叠
摘要
Abstract
Loop-mediated isothermal amplification (LAMP) is a novel method of nucleic acid amplification developed in the last 10 years, with the advantages such as simplicity, rapidity, high sensitivity and high specificity. It is always applied in the detection of pathogenic microbe. This method employs a DNA polymerase with strand displacement activity and a set of 4 different primers specifically designed to recognize 6 distinct regions on the target DNA, with fewer steps and simpler equipment compared to PCR assays. This paper summarized the applications of LAMP method in the detection of pathogenic microbe.关键词
环介导等温扩增(LAMP)/致病菌/检测Key words
LAMP/ pathogenic microbes detection分类
医药卫生引用本文复制引用
贺尔娜,蔡挺,张顺..环介导等温扩增技术在致病菌检测中的应用[J].中国人兽共患病学报,2012,28(7):739-744,6.
