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广东省耐甲氧西林金黄色葡萄球菌SCCmec分型研究

黄革 蒋文玲 黄爱伟 李运雄

中国人兽共患病学报2012,Vol.28Issue(7):745-749,5.
中国人兽共患病学报2012,Vol.28Issue(7):745-749,5.DOI:10.3969/cjz.j.issn.1002-2694.2012.07.020

广东省耐甲氧西林金黄色葡萄球菌SCCmec分型研究

Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus in Guangdong Province, China

黄革 1蒋文玲 2黄爱伟 3李运雄1

作者信息

  • 1. 广东省医学科学院,广东省人民医院检验科,广州510080
  • 2. 广东省医学科学院,广东省人民医院医学研究中心,广州510080
  • 3. 广东省医学科学院,广东省人民医院感染控制医学部,广州510080
  • 折叠

摘要

Abstract

To investigate staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in Guangdong, 3 sets of multiplex PCRs (MPCRs) were used to type SCCmec, and MPCR1 was used for detecting the special loci, common loci, distinctive loci and internal positive control locus in SCCmec. MPCR2 and MPCR3 were used for analyzing the mec gene complex and ccr gene complex, respectively. Results showed that among the 150 MRSA which was typeable, 125 (83. 3%) were Type Ⅲ , 11 (7. 3%) were type Ⅱ , and 7 (4. 7%) were type Ⅳ or Ⅴ. It's definitely that type Ⅲ SCCmer is prevailing in methicillin-resistant Staphylococcus aureus strains isolated in Guangdong Province.

关键词

耐甲氧西林金黄色葡萄球菌/葡萄球菌盒染色体mec/多重PCR/分型

Key words

methicillin-resistant Staphylococcus aureus (MRSA)/ staphylococcal chromosome cassette mec (SCCmec)/ multiplex PCR/ typing

分类

医药卫生

引用本文复制引用

黄革,蒋文玲,黄爱伟,李运雄..广东省耐甲氧西林金黄色葡萄球菌SCCmec分型研究[J].中国人兽共患病学报,2012,28(7):745-749,5.

基金项目

广东省医学科研基金(A2009012) (A2009012)

中国人兽共患病学报

OA北大核心CSCDCSTPCD

1002-2694

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