中国水稻科学2012，Vol.26Issue(4)：476-480,5.DOI:10.3969/j.issn.1001-7216.2012.04.013
OsDUF500基因沉默提高水稻对白叶枯病的抗性
Silencing of OsDUF500 Gene in Rice Enhances Resistance to Xanthomonas oryzae pv.oryzae
摘要
Abstract
Conserved functional region of DUF500 was cloned by PCR from cDNA of expression Harpin*,,, (hrfl ) transgenicrice NJH12. OsDUF500 encodes an unknown function member of DUF500 family. Calli from mature embryos of rice cultivar R109 were transformed by Agrobacterium tumefaciens strain EHA105/ABD: : pVec8. There were eight OsDUF500 transgenic lines from nine hygromycin resistant lines by PCR analysis. Compared with the wild type, the down-expressed lines ABD are characterized by reduced plant height, shortened leaf and white blighted grain. Disease assays of transgenic rice plants infected by Xanthomonas oryzae pv. oryzae (PX099) indicated that different transgenic lines had different resistance to rice leaf blight. Lesion length of the down-expression lines reduced significantly and lesion area all bellowed 30%. Lesion areas of the ABD-3 and ABD-5 were 12. 3% and 15. 7%, respectively. Thus, we predicted OsDUFSOO gene, which was up-regulated in rice blast, maybe was a negative regulator on bacterial blight resistance.关键词
OsDUF500/转基因水稻/白叶枯病/抗性Key words
OsDUFSOO gene/ transgenic rices Xanthomonas oryzae pv. oryzae/ resistance分类
生物科学引用本文复制引用
李娟,常闪闪,刘凤权,邵敏..OsDUF500基因沉默提高水稻对白叶枯病的抗性[J].中国水稻科学,2012,26(4):476-480,5.基金项目
国家863计划资助项目(2008AA10Z108,2007AA10Z188) （2008AA10Z108,2007AA10Z188）
国家转基因生物新品种培育重大专项(2009ZX08001-005B). （2009ZX08001-005B）
