首页|期刊导航|中国神经再生研究（英文版）|Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury
中国神经再生研究（英文版）2012，Vol.7Issue(19)：1445-1453,9.DOI:10.3969/j.issn.1673-5374.2012.19.001
Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury
Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury
摘要
Abstract
Bone marrow mesenchymal stem cells were isolated,purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method.Passages 3-5 bone marrow mesenchymal stem cells were transplanted into rats with traumatic spinal cord injury via the caudal vein.Basso-Beattie-Bresnahan scores indicate that neurological function of experimental rats was significantly improved over transplantation time (1-5 weeks).Expressions of choline acetyltransferase,glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation,determined by immunofluorescence staining and laser confocal scanning microscopy.Bone marrow mesenchymal stem cells that had migrated into the damaged area of rats in the experimental group began to express choline acetyltransferase,glutamic acid decarboxylase and synapsins,3 weeks after transplantation.The Basso-Beattie-Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins.Experimental findings indicate that intravenously transplanted bone marrow mesenchymal stem cells traverse into the damaged spinal cord of rats,promote expression of choline acetyltransferase,glutamic acid decarboxylase and synapsins,and improve nerve function in rats with spinal cord injury.关键词
bone marrow mesenchymal stem cells/spinal cord injury/choline acetyltransferase/glutamic acid decarboxylase/synapsins/neural regenerationKey words
bone marrow mesenchymal stem cells/spinal cord injury/choline acetyltransferase/glutamic acid decarboxylase/synapsins/neural regeneration引用本文复制引用
Shaoqiang Chen,Bilian Wu,Jianhua Lin..Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury[J].中国神经再生研究（英文版）,2012,7(19):1445-1453,9.基金项目
This study was supported by the Doctoral Fund of Ministry of Education of China,No.20060392003 and Academic Development Foundation of Fujian Medical University,No.JS08004. （）
