首页|期刊导航|中国神经再生研究（英文版）|Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
中国神经再生研究（英文版）2012，Vol.7Issue(19)：1483-1487,5.DOI:10.3969/j.issn.1673-5374.2012.19.006
Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
摘要
Abstract
In this study,cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3.After 7 days of culture,immunocytochemical staining showed that,22.4％ of cells were positive for nestin,10.5％ were positive for β-Ⅲ tubulin (neuronal marker),and 60.6％ were positive for glial fibrillary acidic protein,but no cells were positive for O4 (oligodendrocytic marker).At 14 days,there were 5.6％ nestin-,9.6％ β-Ⅲ tubulin-,81.1％ glial fibrillary acidic protein-,and 2.2％ O4-positive cells.In cells not treated with neurotrophin-3,some were nestin-positive,while the majority showed positive staining for glial fibrillary acidic protein.Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.关键词
neurotrophin-3/neural stem cells/differentiation/neuron/oligodendrocytes/stem cells/neural regenerationKey words
neurotrophin-3/neural stem cells/differentiation/neuron/oligodendrocytes/stem cells/neural regeneration引用本文复制引用
Guowei Zhu,Chongran Sun,Weiguo Liu..Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes[J].中国神经再生研究（英文版）,2012,7(19):1483-1487,5.基金项目
This research is supported by the National Natural Science Foundation of China,No.30801181 and a grant by Medical Scientific Research of Zhejiang Province,No.2008B102. （）
