首页|期刊导航|中国神经再生研究（英文版）|Immunotherapy of rat glioma without accumulation of CD4+CD25+FOXP3+ regulatory T cells
中国神经再生研究（英文版）2012，Vol.7Issue(19)：1498-1506,9.DOI:10.3969/j.issn.1673-5374.2012.19.009
Immunotherapy of rat glioma without accumulation of CD4+CD25+FOXP3+ regulatory T cells
Immunotherapy of rat glioma without accumulation of CD4+CD25+FOXP3+ regulatory T cells
摘要
Abstract
Immunotherapy may be used for the treatment of glioblastoma multiforme; however,the induced immune response is inadequate when either T cells or dendritic cells are used alone.In this study,we established a novel vaccine procedure in rats,using dendritic cells pulsed with C6 tumor cell lysates in combination with adoptive transfer of T lymphocytes from syngenic donors.On day 21after tumor inoculation,all the rats were sacrificed,the brains were harvested for calculation of glioma volume,cytolytic T lymphocyte responses were measured by cytotoxic assay,and the frequency of regulatory T lymphocytes (CD4+CD25+FOXP3+) in the peripheral blood was investigated by flow cytometric analysis.The survival rate of rats bearing C6 glioma was observed.Results showed that the co-immunization strategy had significant anti-tumor potential against the pre-established C6 glioma,and induced a strong cytolytic T lymphocyte response in rats.The frequency of peripheral blood CD4+CD25+FOXP3+ regulatory T lymphocytes was significantly decreased following the combination therapy,and the rats survived for a longer period.Experimental findings indicate that the combined immunotherapy of glioma cell lysate-pulsed dendritic cell vaccination following adoptive transfer of T cells can effectively inhibit the growth of gliomas in rats,boost anti-tumor immunity and produce a sustained immune response while avoiding the accumulation of CD4+CD25+FOXP3+ regulatory T lymphocytes.关键词
glioma/dendritic cell/adoptive T cell/combined immunotherapy/CD4+CD25+FOXP3+ regulatory T cellKey words
glioma/dendritic cell/adoptive T cell/combined immunotherapy/CD4+CD25+FOXP3+ regulatory T cell引用本文复制引用
Enshan Feng,Haili Gao,Wei Su,Chunjiang Yu..Immunotherapy of rat glioma without accumulation of CD4+CD25+FOXP3+ regulatory T cells[J].中国神经再生研究（英文版）,2012,7(19):1498-1506,9.基金项目
This work was supported by the grant from the National Natural Science Foundation of China,No.30872647. （）
