中国输血杂志2012，Vol.25Issue(3)：224-227,4.
临沂地区RhD初筛阴性献血人群分子遗传背景研究
The molecular background of RhD negative blood donors in Linyi, China
郭明 1李宏 2宋宁 1陈晓建 1张春来 2张霞 2陈强 2陈静娴1
作者信息
- 1. 中国医学科学院输血研究所,四川成都610052
- 2. 临沂市红十字会中心血站
- 折叠
摘要
Abstract
Objective To elucidate the molecular background of RhD variant blood donors in Linyi. Methods A total of 54 458 blood samples from random blood donors were collected from January to October in 2011. RhD were tested by saline method,and 241 RhD negative samples. Genomic DNA was extracted from the RhD negative specimens identified by serological testing. RHD genoryping was performed by using polymerase chain reaction with sequence-specific primers (SSP-PCR). Results The RhD negative rate was 0.44%. Among 241 RhD negative samples, there were 169 samples with deletion of 1 ~ 10 exons, 22 with deletion of 2 ~9 exons, 38 with 1227G > A mutation, 5 with 845 G > A mutation,5 with deletion of 3 ~6 exons, 1 with RHD(711DelC) ,1 with Dva(Hus) ,A total of 38 Del samples were found using molecularbiolo-gy methods, and the positive rate was 15. 77% (38/241). Conclusion Among the blood donors of linyi area,preliminary screening shows RHD gene polymorphism was existed by preliminary screening. The rate of RhDel is an important genetic marker for RhDel phenotype in LinYi, China.关键词
RhD阴性/Del型/RHD 1227A等位基因Key words
RhD negative/ Del/ RHD 1227 A分类
临床医学引用本文复制引用
郭明,李宏,宋宁,陈晓建,张春来,张霞,陈强,陈静娴..临沂地区RhD初筛阴性献血人群分子遗传背景研究[J].中国输血杂志,2012,25(3):224-227,4.
