摘要
Abstract
Objective To evaluate the effect of trehalose-loading RBCs on the lyophilization and the effect of different extracellular trehalose concentration on the lyophilization of trehalose-loaded RBCs. Methods RBCs was incubated in 800mmol/L concentration of trehalose solution at 371 for7 hours, and RBCs incubated in phosphate-buffered saline were used as control. Trehalose loading red blood cells and solution of different extracellular trehalose concentration were mixed at ratio of 1 : 1, Samples were lyophilizated, the recovery rate of hemoglobin was detected by using cyanohemeglobin kit. Intracellular ATP was determined by bioluminescence assay. Results The recovery rate of hemoglobin in trehalose-loading RBC was(44.46 ±5. 15)% ,the level of ATP( 1. 91 ±0.13)μmol/gRb, the recovery rate of hemoglobin in contra! Was(7. 71 ±2.71)% ,the level of ATP(0. 88 ±0. 25) μmol/gHb, Comparison of recovery of hemoglobin and level ATP of lyphilizated RBC show significantly different for P <0. 005. The recovery rate of hemoglobin in 50 mmol/L group was(33. 57 ±2. 89)% ,in 200mmol/L group was(38. 64 ±0.54)% ,in 400nunol/L group was(18. 10 ± 1. 95)% ,while The recovery rate of hemoglobin in control group was (10. 36 ± 0. 97) %. Comparision of recovery of hemoglobin of lyphilizateg RBC with solution of different extracellular trehalose cencontration ,the data of recovery of hemoglobin of PBS( control group) show significantly different for P<0.001 with50 mmol/L group, 200mmol/L group, 400mmol/L group. Compnrision of recovery of hemoglobin of 400mmol/L group with 50mmol/L group, 200mmol/L group, data show significantly different for P <0.001. 50 mmol/L group, 200mmol/L group, 400ramol/L group were significantly higher than that of PBS group, 50mmol/ L group, 200mmol/L group were significantly higher than that of 400mmol/L group. 50mmol/L group was not significantly different with 200mmol/L group. Conclusion The recovery of hemoglobin and the level of ATP is improved in lyophiliza-tion of trehalose-loaded RBCs and the extracellular trehalose has protective effect, which decreased with increasing of extracellular trehalose concentrations beyond 200mmol/L关键词
红细胞/海藻糖/冷冻干燥保存Key words
RBC/ Trehalose/ Lyophilization分类
医药卫生
