中国畜牧兽医2012，Vol.39Issue(5)：41-43,3.
鸡蛔虫线粒体cox1基因的克隆及序列分析
Cloning and Sequence Analysis of Mitochondrial cox1 Gene of Ascaridia galli Isolates from Different Provinces in China
摘要
Abstract
The objectives of the present study were to analyze sequence variations in the cytochrome c oxidase subunit 1 (coxl) gene among Ascaridia galli isolates from different provinces in China,and to study its phylogenetic relationships with roundworms of the other families using coxl gene sequence. The partial coxl (pcoxl) were amplified from each A. galli sample,and pcoxl sequences were aligned using the Clustal X 1. 81. MP and NJ trees were constructed using the software Phylip 3. 67 version 4. 0 and Mega version 4. O.and ML tree was also constructed using Puzzle version 5. 2. The lengths of all the pcoxl sequences were 250 bp. Phylogenetic analyses showed that the eight isolates were clustered in the same clade. Sequence variations in pcoxl sequences within A. galli were 0 to 2. 5% which was significantly lower than inter-species differences (6. 9% to 15. 3%). It is concluded that pcoxl sequences can be used as genetic marker for population genetic studies of A. galli. The results of this study provided foundation for further studies on molecular genetics and diagnostics of A. galli.关键词
鸡蛔虫/线粒体DNA/cox1基因/种系发育关系Key words
Ascaridia galli/ mitochondrial DNA/ coxl gene/ phylogenetic relationship分类
生物科学引用本文复制引用
李佳缘,刘国华,王燕,林宇,罗健,付汉冲,周东辉..鸡蛔虫线粒体cox1基因的克隆及序列分析[J].中国畜牧兽医,2012,39(5):41-43,3.基金项目
家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2009KFKT014、SKLVEB2010KFKT010、SKLVEB2011KFKT004) （SKLVEB2009KFKT014、SKLVEB2010KFKT010、SKLVEB2011KFKT004）
教育部“长江学者和创新团队发展计划”创新团队项目(IRT0723)资助. （IRT0723）
