中国畜牧兽医2012，Vol.39Issue(5)：52-54,3.
中华双腔吸虫线粒体cox1基因的克隆及序列分析
Cloning and Sequence Analysis of Mitochondrial cox1 Gene of Dicrocoelium chinensis
摘要
Abstract
The objectives of the present study were to amplify and analyse the mitochondrial cytochrome c oxidase subunit 1 {coxl} gene among D. ckinensis isolates from Gansu province in China, and to study its phylogenetic relationships with trema-todes of the other families using coxl gene sequence. The partial coxl (pcoxl) were amplified from each D. chinensis sample, and pcaxl sequences were aligned using the Clustal X 1. 81. Maximum parsimony (MP) and maximum likelihood (ML) trees were constructed using the software Phylip 3. 67 version 4.0, and ML tree was also constructed using Puzzle version 5. 2. The lengths of all the pcaxl sequences were 355 bp. Phylogenetic analyses showed that the D, chinensis samples from Gansu were clustered in the same clade. The present study reported the mitochondrial coxl sequence of D. chinensis for the first time. The results of this study provided foundation for further studies on molecular genetics and diagnostics of D. chinensis.关键词
中华双腔吸虫/线粒体DNA/cox1基因/种系发育关系Key words
D. chinensis/ mitochondrial UNA/coxl gene/relationship分类
生物科学引用本文复制引用
王燕,刘国华,李佳缘,林少淇,林泽萍,宋此伊,周东辉..中华双腔吸虫线粒体cox1基因的克隆及序列分析[J].中国畜牧兽医,2012,39(5):52-54,3.基金项目
国家肉牛牦牛产业技术体系项目(CARS-38) （CARS-38）
家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2009KFKT014、 SKLVEB2010KFKT010、SKLVEB2011KFKT004)资助. （SKLVEB2009KFKT014、 SKLVEB2010KFKT010、SKLVEB2011KFKT004）
