中国畜牧兽医2012，Vol.39Issue(5)：55-59,5.
延边黄牛白细胞介素18基因的克隆及序列分析
Cloning and Sequence Analysis of Yanbian Bovine Interleukin 18
摘要
Abstract
The aim of this paper was to amplify full length cDNA genes of Yanbian BoIL-18 and make phylogenetic anylys-is. Designing and synthesizing one pair of primers according to bovine IL-18 gene sequences published in GenBank. Bovine IL-18 gene was amplified by RT-PCR from bovine splenic lymphocyte which stimulating by both ConA and PHA for 36 h,then was cloned,double restriction digested and sequenced to identify the positive samples,further to carry out phylogenetic analysis and secondary structure prediction. Having amplified full length cDNA genes of 760 bp of Yanbian BoIL-18,there were two amino acid different. Phylogenetic analysis showed that Yanbian BoIL-18 had high homology with Bos taurus,Capra hircus,Ovis ari-es ,Equus caballus ,Sus scrofa ,Canis lupus and had low homology with Gallusgullus (51.1%). We make sure the group differences of IL-18 and predicted the secondary structure,laying a foundation for further expressing bovine IL-18, biological activity and utilized as a adjuvant.关键词
牛白细胞介素/RT-PCR/进化分析Key words
bovine interleukin 18/ RT-PCR /phylogenetic analysi分类
生物科学引用本文复制引用
张立霞,高旭,宋建臣,孙平杰,夏晓辉,许应天..延边黄牛白细胞介素18基因的克隆及序列分析[J].中国畜牧兽医,2012,39(5):55-59,5.基金项目
延边大学“211”工程三期重点学科建设项目. （）
