中国畜牧兽医2012，Vol.39Issue(5)：126-129,4.
IGF-Ⅰ在奶牛乳腺的表达及其对乳腺上皮细胞泌乳功能的影响
Expression of IGF-Ⅰ in Mammary Gland and the Effects of IGF-Ⅰon Mammary Epithelial Cells
摘要
Abstract
To explore the relationship between expression of IGF- I and development and lactation ability of dairy cow mammary gland, the expression of IGF-Ⅰ in dairy cow mammary tissue was detected using qRT-PCR. The cell culture, qRT-PCR, and MTT assay were also used to test the effects of IGF-Ⅰ on dairy cow mammary epithelial cells. The results showed that expression of IGF-Ⅰ in mammary tissue in puberty cow was the highest. After IGF-Ⅰ addition, IGF-Ⅰ R expression, proliferation ability of mammary epithelial cells and beta casein expression were raised. The results suggest that IGF- I probably promotes IGF-Ⅰ R expression, further encourages the proliferation of mammary epithelial cells and increases beta casein content.关键词
奶牛/IGF-Ⅰ/IGF-Ⅰ R/表达/泌乳Key words
dairy cow/IGF-Ⅰ/ JGF-Ⅰ R/expression/lactation分类
生物科学引用本文复制引用
张童非,崔英俊,李庆章..IGF-Ⅰ在奶牛乳腺的表达及其对乳腺上皮细胞泌乳功能的影响[J].中国畜牧兽医,2012,39(5):126-129,4.基金项目
国家重点基础研究发展计划(2011CB100804) （2011CB100804）
东北农业大学创新团队计划(CXT005-1-1/-2)资助. （CXT005-1-1/-2）
