中国畜牧兽医2012，Vol.39Issue(5)：160-164,5.
梅山与杜洛克母猪发情前期卵巢组织差异表达基因的分离与鉴定
Research on the Differentially Expressed Genes between the Ovarian Tissues of Meishan and Duroc Sows at Proestrus Stage
摘要
Abstract
In order to reveal the effects on ovulation and reproduction traits, differentially expressed genes in pig ovaries was detected. We performed the mRNA differential diplay (DD) technique to investigate the differences in gene expression in the ovary tissues from Meishan and Duroc. Nine expressed sequence tags (ESTs), differentially expressed between Meishan and Duroc, were isolated and identilied through semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The nucleotide sequence analysis revealed that the 3 ESTs showed similarity to known genes published in GenBank. Tissue expression profile analysis showed that the ESTs were expressed differently in heart, liver, lung, kidney, muscle, fat (subcutaneous fat little legs, the same below) , small intestine, brain, ovary, uterus, spleen, oviduct. The results indicated the diversity of differential display direction of genes between Meishan and Duroc. The results also suggested that the differentially expressed genes will result in the differences of reproductive traits in pigs and provide the basic research for further study on the molecular mechanism of ovulation regulatory network.关键词
梅山猪/杜洛克猪/卵巢/半定量/组织表达谱Key words
Meishan/ Duroc/OvaryiSemi-quantitative/tissue expression profiles分类
生物科学引用本文复制引用
赵志超,孙敬礼,黄涛,李大全,王宵燕,宋成义,刘丽娟,杨飞..梅山与杜洛克母猪发情前期卵巢组织差异表达基因的分离与鉴定[J].中国畜牧兽医,2012,39(5):160-164,5.基金项目
石河子大学项目(RCZX200602) （RCZX200602）
国家自然基因(30901014). （30901014）
