中国畜牧兽医2012，Vol.39Issue(5)：192-195,4.
牛副结核病PCR检测方法的建立
Establishment of PCR for Detecting Bovine Paratuberculosis
摘要
Abstract
A pair of primers were designed according to the sequence of specific Mycobacterium paratuberculosis gene of C-2 chromosotne(ISMav2), the ISMav2 gene was amplified by PCR and cloned into pMD18-T vector and then the gene sequence was detected. The results showed that the target gene band at a length 246 bp was amplified by PCR, with a homology of 99. 6% to the gene sequence reported in GenBank. The experiments had proved that PCR assay possessed a high specificity, DNA bands couldn't be amplified in other bacterial except Mycobacterium paratuberculosis. And the sensitivity test results indicated that PCR assay was more sensitive, which could detect ISMav2 with only 1 pg DNA. The successfully construction of the PCR assay provides strongly technical support for detection,identification and epidemiological investigations of bovine paratuberculosis.关键词
牛/副结核分枝杆菌/PCR/检测方法Key words
bovine/ MP/PCR/ detection分类
农业科技引用本文复制引用
王素华,王忠才,李孝军,杜爱芳..牛副结核病PCR检测方法的建立[J].中国畜牧兽医,2012,39(5):192-195,4.基金项目
浙江出入境检验检疫局立项项目(2011ZK019). （2011ZK019）
