中国畜牧兽医2012，Vol.39Issue(7)：47-52,6.
高致病性猪繁殖与呼吸综合征病毒山西分离株的鉴定及部分Nsp2基因序列分析
Analysis of Sequence of Nsp2 Gene Isolated from Highly Pathogenic PRRSV Strains in Shanxi
摘要
Abstract
In this study, total RNA was extracted from tissues and serum in incident swines from different regions of Shanxi. The highly pathogenic PRRSV of 16 samples were positive by RT-PCR. The gene sequence of Nsp2 was amplified using the specific primers. The analysis of gene sequences by DNAStar and bioinfomatic software showed that the amino acids sequence of Nsp2 shared 69. 8% to 71. 7%, 85. 3% to 87.9%, 68.7% to 70. 6%, 91. 1% to 94.3%, 94.3% to 98.5%, 95. 1% to 98. 9% similarity with VR-2332, CH-la, BJ-4, HB-1, HuN4 and JXA1 strains respectively, following the comparison of sequences and homologue analysis. The results also showed that Nsp2 from Shanxi strains lacked amino acids at 2 locations, and one location was 1 amino acid and another was continuous 29 amino acids. It suggested that the PRRSV mutant strains in Shanxi belonged to the same branch as the domestic epidemic strains.关键词
高致病性猪繁殖与呼吸综合征病毒/分离与鉴定/Nsp2基因/序列分析Key words
PRRSV/solation and identification/Nsp2 gene/sequence analysis分类
生物科学引用本文复制引用
吴忻,樊振华,米瑞娟,韩一超,王娟萍,刘文俊,姚敬明,孟帆,武守艳,雷宇平,陈剑波..高致病性猪繁殖与呼吸综合征病毒山西分离株的鉴定及部分Nsp2基因序列分析[J].中国畜牧兽医,2012,39(7):47-52,6.基金项目
山西省科技攻关项目(20090311034). （20090311034）
