中国畜牧兽医2012，Vol.39Issue(7)：58-61,4.
牛支原体单克隆抗体的制备与鉴定
Preparation and Identification of Monoclonal Antibodies against Mycoplasma bovis
摘要
Abstract
8-week-old female BALB/c mice were immunized with Mycoplasma bovis HB0801. By using the hybridoma technique, six hybridoma cell lines secreting monoclonal antibodies (MAbs) were identified. The ascite for the six MAbs were prepared and the characteristics of them were further studied. All of the six MAbs belonged to IgG category. The antibody liters of these MAbs ranged from lX105 to 1. 6×106. The specificity of these MAbs was detected by ELISA. The result suggested that all these MAbs can react with Mycoplasma bovis strains clinically isolated from China and the ATCC reference strain PG45, but they did not react with other bovine pathogenic bacteria tested such as Pasteurella multocida, Arcanobacterium pyogenes and so on. All MAbs investigated cross-reacted with Mycoplasma agalactiae which was known to be closely related to Mycoplasma bovis, prevalent in goats but not in cattle. Among them, two MAbs 1A5 and 1C11 did not exhibit further cross-reactions to other Mycoplasma species tested. In addition, Western blotting assay demonstrated that these six antibodies recognized various Mycoplasma bovis proteins. In conclusion, the MAbs prepared in this study layed the foundation for the establishment of novel detection methods for Mycoplasma bovis and studying its pathogenesis.关键词
牛支原体/单克隆抗体/免疫印迹/ELISA/杂交瘤/特异性Key words
MycopUisma bovis/ monoclonal antibodies (MAbs)/ Western blotting/ ELISA/ hybridoma/ specificity分类
农业科技引用本文复制引用
任泽民,姜勇,巴晓亮,胡长敏,陈颖钰,彭清洁,陈焕春,郭爱珍..牛支原体单克隆抗体的制备与鉴定[J].中国畜牧兽医,2012,39(7):58-61,4.基金项目
农业公益性行业科研项目(201003060):现代农业(肉牛)产业技术体系专项资金(CARS-38). （201003060）
