中国畜牧兽医2012,Vol.39Issue(7):62-65,4.
变性梯度凝胶电泳技术及其在动物肠道菌群研究中的应用
Review on Denaturing Gradient Gel Electrophoresis and its Application in Animal Intestinal Bacterial Community
摘要
Abstract
Denaturing gradient gel electrophoresis (DDGE) was a new electrophoresis method for testing the nucleic acid variation and point mutations, mainly based on the different denaturing concentrations dued to the different nucleic acid sequence of mutant type and wild type, thus using the denaturing,gradient glue to make it separate. It could be used in the diversity and dynamics analyses of microbial communities. The principles of DGGE, applications in diversity and dynamics of intestinal bacterial community, the limitations and future developing perspectives were summarized in this paper.关键词
变性梯度凝胶电泳/肠道菌群/多样性/动态变化Key words
denaturing gradient gel electrophoresis/ intestinal bacterial community) diversity/ dynamics分类
生物科学引用本文复制引用
赵春苗,徐春厚..变性梯度凝胶电泳技术及其在动物肠道菌群研究中的应用[J].中国畜牧兽医,2012,39(7):62-65,4.基金项目
广东省科技计划项目(2008B020800007). (2008B020800007)