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变性梯度凝胶电泳技术及其在动物肠道菌群研究中的应用

赵春苗 徐春厚

中国畜牧兽医2012,Vol.39Issue(7):62-65,4.
中国畜牧兽医2012,Vol.39Issue(7):62-65,4.

变性梯度凝胶电泳技术及其在动物肠道菌群研究中的应用

Review on Denaturing Gradient Gel Electrophoresis and its Application in Animal Intestinal Bacterial Community

赵春苗 1徐春厚1

作者信息

  • 1. 广东海洋大学动物医学系,广东湛江524088
  • 折叠

摘要

Abstract

Denaturing gradient gel electrophoresis (DDGE) was a new electrophoresis method for testing the nucleic acid variation and point mutations, mainly based on the different denaturing concentrations dued to the different nucleic acid sequence of mutant type and wild type, thus using the denaturing,gradient glue to make it separate. It could be used in the diversity and dynamics analyses of microbial communities. The principles of DGGE, applications in diversity and dynamics of intestinal bacterial community, the limitations and future developing perspectives were summarized in this paper.

关键词

变性梯度凝胶电泳/肠道菌群/多样性/动态变化

Key words

denaturing gradient gel electrophoresis/ intestinal bacterial community) diversity/ dynamics

分类

生物科学

引用本文复制引用

赵春苗,徐春厚..变性梯度凝胶电泳技术及其在动物肠道菌群研究中的应用[J].中国畜牧兽医,2012,39(7):62-65,4.

基金项目

广东省科技计划项目(2008B020800007). (2008B020800007)

中国畜牧兽医

OA北大核心CSTPCD

1671-7236

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