中国畜牧兽医2012，Vol.39Issue(8)：16-19,4.
靶向env及LTR基因的siRNA抑制ALV-J复制的研究
siRNAs Targeted to env and LTR Gene for Inhibition of Avian Leukosis Virus Subgroup J Replication
摘要
Abstract
According to the env and LTR gene of avian leukosis virus subgroup J, 4 and 5 short hairpin RNAs (shRNAs) targeting their conserved sequences were designed systhessised and cloned into pSilencer 4. 1 vector respectively. The recombi-nant plasmids were transfected into DF-1 cell and infected with 103 TCID50 ALV-J at 6 hours post-transfection, the efficiency of RNA interfering was assayed by Real-time RT-PCR . The results revealed that the recombinant plasmids of pSi4. 1-env inhibited virus growth by 18. 15% to 63. 43% and pSi4. 1-LTR by 19. 37% to 45. 41% compared to negative control.关键词
J亚群禽白血病病毒/env基因/LTR基因/siRNAKey words
avian leukosis virus subgroup J virus / env genet LTR gene/siRNA分类
农业科技引用本文复制引用
李娇,冯少珍,黄武,吴晓婵,李育豪,曹伟胜,廖明..靶向env及LTR基因的siRNA抑制ALV-J复制的研究[J].中国畜牧兽医,2012,39(8):16-19,4.基金项目
NSFC-广东联合基金(U0831002) （U0831002）
国家肉鸡产业技术体系项目(nycytx-42-G3-03) （nycytx-42-G3-03）
广东省自然科学基金项目(S2011010001946 （）
10451064201005432). （）
