中国畜牧兽医2012，Vol.39Issue(8)：73-76,4.
昆明鼠白细胞介素-15基因的克隆与序列分析
Cloning and Sequence Analysis of IL-15 Gene of Chinese KM Mice
摘要
Abstract
According to IL-15 gene sequence of Mus musculus available in GenBank database, specific primers were designed and synthesized. Using reverse transcription-polymerase chain reaction (RT-PCR) method, mature IL-15 sequence was amplified from the spleen cells of Kunming (KM) mice stimulated for 72 h by Toxoplasma gondii RH strain, then cloned into pMD18-T vector and transformed into the competent Escherichia coli JM109 for obtaining the positive clone and sequenced. Sequencing analysis revealed that the length of mature IL-15 of KM mice was 345 bp, encoding 114 amino acids. The results of sequence alignment and genetic evolution analysis showed that the KM mice mature IL-15 sequence was the closest to Norvegi-cus rattus , and the homologies of nucleotide and amino acid sequence were 94. 2% and 95. 5% respectively. Sequence similarity between the KM mice mature IL-15 sequence and that of Cavia porcellus , human, primates, Oryctolagus cuniculus, dog, cat. Bos taurus, Sus scrofa etc. , and the nucleotide hotnology ranged between 71.2% to 78. 8% , and the amino acid homology was 69. 5% to 74. 7%. The successful cloning of KM mouse IL-15 gene provided foundation for further studies of its functions and immune stimulating effects.关键词
KM鼠/IL-15/克隆/进化分析Key words
KM mice/IL-15/cloning/evolution analysis分类
生物科学引用本文复制引用
李中原,李佳缘,周东辉,朱兴全,陈佳..昆明鼠白细胞介素-15基因的克隆与序列分析[J].中国畜牧兽医,2012,39(8):73-76,4.基金项目
家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2010KFKT009、SKLVEB2011KFKT010、SKLVEB2009KFKT008、SKLVEB2011KFKT004). （SKLVEB2010KFKT009、SKLVEB2011KFKT010、SKLVEB2009KFKT008、SKLVEB2011KFKT004）
