中国畜牧兽医2012，Vol.39Issue(8)：136-138,3.
不同消化酶对羊驼皮肤黑素细胞分离的影响
Effects of Different Enzymes on the Melanocyte Isolation of Alpaca Skin
摘要
Abstract
In order to explore and establish a new method of isolation,culture,and identification of alpaca skin melanocyte in vitro for further studying to provide a suitable pigment deposition cell model. Take 1 to 3 years young alpacal skin, with three different enzymes (trypsin,trypsin-EDTA .Dispase Ⅱ) digestion alpaca skin,alpaca melanocyte were obtained by trypsin-EDTA digestion,placed into DMEM/F12 culture medium. Analyze melanocyte growth,cell yields and cells attached. The results snowed Dispase Ⅱ group had a significant higher other groups (P<0. 01). In summary.alpaca melanocyte were obtained by Dispase Ⅱ digestion.关键词
羊驼/黑素细胞/DispaseⅡ酶/纯化Key words
alpaca/melanocyte/Dispase Ⅱ /depuration分类
生物科学引用本文复制引用
白瑞,于志慧,杨刚,王海东,范瑞文,董常生..不同消化酶对羊驼皮肤黑素细胞分离的影响[J].中国畜牧兽医,2012,39(8):136-138,3.基金项目
国家自然基金(30571070,30671512) （30571070,30671512）
山西农业大学科技创新基金(2011002). （2011002）
