中国畜牧兽医2012，Vol.39Issue(8)：165-170,6.
中国荷斯坦牛PPAR-α基因多态性研究及其与耐热性能的关联分析
Study on the Polymorphisms of PPAR-α Gene and its Association with Heat Shock Response in Chinese Holstein
摘要
Abstract
In this study, we detected a novel single-nucleotide polymorphism of the PPAR-α gene in Chinese native cattle by screening for genetic variation of PPAR-a in 944 individuals of three Chinese cattle breeds,namely China Holstein, Bohai Black and Luxi Yellow,using PCR-RFLP and DNA sequencing techniques. The sequencing results indicated that there was one single nucleotide mutation (G to A) in the 44087 of the PPAR-α gene. Chi-square test indicated that 44087GT polymorphic site in the PPAR-a gene didn't meet Hardy-Weinberg equilibrium in Holstein and Luxi cattle(P<0. 05) ;The genetic correlations between genotypes and heat shock response were obtained by the least square analysis,and the results showed that genetic polymorphisms significantly correlated with somatic cell scores (SCS).and milk productive traits. GA genotype in 44087G>A polymorphic site in PPAR-a gene was an excellent site.SCS of the individuals were significantly lower than GG genotype (P< 0. 05); cows with GA genotype had a significantly higher protein levels and milk yield in the hot environment than GG genotype (P<0. 05) ,so GA genotype was resistive heat. The resulting analysis of the GA genotype might help to improve the performance of Chinese Holstein cows milk. In the process of artificial selection,the GA genotype individuals could reduce heat stress to cows harm,and at the same time was able to improve milk quality and yield.关键词
中国荷斯坦牛/SNPs/PPAR-α基因/多态性/PCR-RFLPKey words
Chinese Holstein /SNPs/PPAR-α gene/polymorphism/PCR-RFLP分类
农业科技引用本文复制引用
方文良,李秋玲,黄金明,王长法,鞠志花,李建斌,李荣岭,李琳,仲跻峰,何剑斌..中国荷斯坦牛PPAR-α基因多态性研究及其与耐热性能的关联分析[J].中国畜牧兽医,2012,39(8):165-170,6.基金项目
国家转基因重大专项(2008ZX08007-004) （2008ZX08007-004）
国家自然基金(31000543) （31000543）
山东省自然科学基金项目(Y2008D56) （Y2008D56）
现代农业产业技术体系建设项目(nycytx-0107) （nycytx-0107）
公益性行业科研专项(nyhyzx07-036-39)资助. （nyhyzx07-036-39）
