中国畜牧杂志2012，Vol.48Issue(15)：18-22,5.
应用ICSI技术生产转基因水牛胚胎的初步研究
Primary Study on Production of Transgenic Buffalo Embryo by ICSI
摘要
Abstract
Effects of sperm membrane and sperm membrane damaging methods on efficiency of transgenesis by ICSI in water buffalo were investigated. The results show that: When sperm membrane was disrupted by freeze-thawing method, the EGFP expressing rate was 21.8% significantly higher than that of live sperm 5.1% (P<0.01), while the cleavage rate, blastocyst development rate was no remarkable difference (P>0.05). When sperm membrane was disrupted by the freeze-thawing, Triton x-100 and sonication respectively, the blastocyst development rate of freeze -thawing group was significantly higher than Triton x-100 group's (16.7% vs. 3.1%, P<0.01). And the EGFP expressing rate of freeze-thawing group was significantly higher than that of the other two groups (60.3% vs. 31.7% vs. 18.2%, P<0.05). These results indicate that, the EGFP expressing embryos of water buffalo could be generated by ICSI method; the efficiency of transgenesis by ICSI in buffalo can be improved by disrupted sperm membrane through freeze-thawing.关键词
ICSI/转基因/水牛Key words
ICSI/ transgenesis/ buffalo分类
农业科技引用本文复制引用
陈自洪,孟凡丽,刘玉兵,陆凤花,王丹,崔奎青,石德顺..应用ICSI技术生产转基因水牛胚胎的初步研究[J].中国畜牧杂志,2012,48(15):18-22,5.基金项目
国家高技术研究发展计划(863)项目(2011AA100607) （863）
国家转基因重大专项(2011ZX08007-003) （2011ZX08007-003）
