中国畜牧杂志2012，Vol.48Issue(17)：1-6,6.
绵羊催乳素受体基因外显子10多态性及其与产羔数相关分析
Analysis on Genetic Polymorphism of Exon 10 of Prolactin Receptor Gene and Its Relationship with Litter Size of Sheep
摘要
Abstract
Prolactin receptor (PRLR)gene was studied as a candidate gene for the prolificacy of Hu sheep. Primers were designed to detect single nucleotide polymorphisms of exon 10 of PRLR gene in Hu sheep, Small Tail Han sheep, Wadi sheep and Altay sheep by PCR-SSCP. The products amplified by P2 of the four sheep and P3 of Altay sheep were accorded with Hardy -Wenberg equilibrium, and others were in Hardy-Wenberg disequilibrium. In the fragment amplified by primer P2 there was no significant difference for the distribution frequency among different genotypes of the four sheep breeds (P> 0.05). In the fragment amplified by primer P3 there was extremely significant difference for the distribution frequency between Altay sheep and the other three breeds (P<0.01), and there were no significant differences among other three breeds (P> 0.05). There were extremely significant difference for the litter size among four breeds(P<0.01), but no significant difference was observed among different genotypes amplified by primer P2 and primer P3 (P>0.05). There was no significant difference among the different genotypes for the litter sizes of Hu sheep (P>0.05). The research showed that PRLR gene couldn't be selected as a candidate gene for the prolificacy of Hu sheep.关键词
催乳素受体基因/多态性/产羔数/绵羊Key words
PRLR gene/ polymorphism/ litter size/ sheep分类
农业科技引用本文复制引用
张向楠,孙伟,储明星,张有法,陈玲,吴文忠,周洪,徐厚生..绵羊催乳素受体基因外显子10多态性及其与产羔数相关分析[J].中国畜牧杂志,2012,48(17):1-6,6.基金项目
现代肉羊产业技术体系建设(CARS-39) （CARS-39）
中国博士后科学基金特别资助项目(200902154) （200902154）
科技部家养动物种质资源平台 （）
江苏省农业科技支撑计划项目(BE2012331) （BE2012331）
江苏高校优势学科建设工程资助项目 （）
国家高技术研究发展计划(863计划)(2011A A100307-04) （863计划）
江苏省苏北科技发展计划(BN2010004) （BN2010004）
