中国烟草学报2012，Vol.18Issue(2)：12-16,5.DOI:10.3969/j.issn.1004-5708.2012.02.003
外源钙对二氯喹啉酸胁迫下烤烟叶片生理特性的影响
Effects of exogenous calcium on physiological characters of flue -cured tobacco under quinclorac stress
摘要
Abstract
Effects of different concentrations of calcium chloride solutions on activity of cellulose, IAAOase, ATPase, content and SDS - PAGE of soluble protein of tobacco under quinclorac stress were investigated with pot experiments. Results showed that, compared with healthy tobacco, activity of cellulose and IAAOase of quinclorac stressed tobacco increased , while activity of ATPase and content of soluble proteins decreased. Spraying calcium chloride solutions with different concentrations on quinclorac coercion tobacco reduced activity of cellulose and IAAOase but increased ATPase activity and content of soluble proteins. Discontinuous SDS - PAGE of soluble proteins revealed that proteins with molecular weights of 50 kDa and 56 kDa in were disappeared when quinclorac was added to soil. Spraying calcium chloride solution resulted in protein with molecular weight of 50 kDa reappeared. A new protein with molecular weight of 120kDa was appeared after 15 days of using exogenous calcium. Results also showed that spraying exogenous calcium had positive effect of the restoration of quinclorac coercion tobacco with the best concentration of 10 mmol/L.关键词
烤烟/叶片/二氯喹啉酸/Ca2+/生理特性Key words
flue-cured tobacco/ leaf/ quinclorac/ Ca2+ / property分类
农业科技引用本文复制引用
刘华山,王晓军,韩锦峰,张志勇,袁士豪,易建华..外源钙对二氯喹啉酸胁迫下烤烟叶片生理特性的影响[J].中国烟草学报,2012,18(2):12-16,5.基金项目
湖南中烟工业有限责任公司科技项目(2009160507) （2009160507）
