中国烟草学报2012，Vol.18Issue(2)：65-69,5.DOI:10.3969/j.issn.1004-5708.2012.02.013
蜡样芽孢杆菌(Bacillus cereus)筛选鉴定及在雪茄烟叶发酵中的应用研究
Isolation and identification of bacillus cereus and its application in cigar leaf fermentation
摘要
Abstract
One bacterial strain named x-2 was isolated from tobacco leaves and was identified belonging to Bacillus cereus on the basis of 16S rRNA gene sequencing and biochemical properties. X-2 pure culture was applied in artificial fermentation of cigar leaf, and thereafter determination of general nitrogen compound and sensory quality evaluation were carried out. Results showed that after fermentation total nitrogen, protein, total alkaloid, and volatile were decreased by 21.64% , 13.53% , 36.38% and 24.05% , respectively. Smoking tests implied that x-2 pure culture may cause more pleasing aroma while less undesirable tastes and bitterness.关键词
蜡样芽孢杆菌(Bacillus cereus)/雪茄烟叶/人工发酵Key words
bacillus cereus/cigar leaf/artificial fermentation分类
轻工纺织引用本文复制引用
李宁,寇明钰,刘林,戴亚,张燕,汪长国,曾代龙,刘一兵,杨军,雷金山,贾玉红,赵敏,吴艳..蜡样芽孢杆菌(Bacillus cereus)筛选鉴定及在雪茄烟叶发酵中的应用研究[J].中国烟草学报,2012,18(2):65-69,5.基金项目
国家高新技术研究发展计划(863计划)(2010AA101506-6) （863计划）
中国烟草总公司重点项目(110201002013) （110201002013）
四川省立项项目(2009JY0156) （2009JY0156）
川渝中烟博士后基金项目(2010-01) （2010-01）
