中国烟草学报2012，Vol.18Issue(3)：6-9,4.DOI:10.3969/j.issn.1004-5708.2012.03.002
卷烟添加木犀草素与黄芩苷混合物对细胞损伤的减害作用研究
Effects of adding Luteolin and Baicalin mixture in cigarettes on reducing cell injury
陈义坤 1魏敏 1宋旭艳 1罗诚浩 1李文芳 2王君 2胡霞敏2
作者信息
- 1. 湖北中烟工业有限责任公司技术中心,武汉市东西湖区金山大道1355号黄鹤楼科技园,430040
- 2. 武汉科技大学医学院,武汉430065
- 折叠
摘要
Abstract
Sample cigarettes were made by adding luteolin and baicalin mixture into cigarettes. Cell cytotoxicity experiments were conducted. Cigarette smoke extract (CSE) was collected by dimethyl sulfoxide (DMSO). Human lung ade-nocarcinoma A549 cells were cultured and then incubated with different concentrations of CSE. Cell viability was evaluated by MTT assay and DNA damage was detected by Comet assay. Contents of reactive oxygen species (ROS) was examined by fluorescence method. Results showed that the sample cigarettes resulted in higher survival rate of A549 cells, lower ROS tail length, tail DNA content, TM, and OTM than control group. It implied that adding luteolin and baicalin mixture into cigarettes could attenuate cell injury, increase cell viability, and reduce DNA damage.关键词
卷烟烟气提取物/木犀草素/黄芩苷/人肺泡上皮细胞A549/细胞内活性氧/DNA损伤Key words
cigarette smoke extract (CSE)/ luteolin/ baicalin/ human lung adenocarcinoma A549 cells/ ROS/ DNA damage分类
轻工纺织引用本文复制引用
陈义坤,魏敏,宋旭艳,罗诚浩,李文芳,王君,胡霞敏..卷烟添加木犀草素与黄芩苷混合物对细胞损伤的减害作用研究[J].中国烟草学报,2012,18(3):6-9,4.
