中国烟草学报2012，Vol.18Issue(3)：10-15,6.DOI:10.3969/j.issn.1004-5708.2012.03.003
洗涤剂法测定烟草及烟草制品中中性洗涤纤维、酸性洗涤纤维、酸性洗涤木质素的研究
Determination of NDF,ADF and ADL in tobacco and tobacco products with detergent method
杨斌 1殷引 1张浩博 1佘永桢 1沈轶1
作者信息
- 1. 上海烟草集团有限责任公司技术中心,上海市杨浦区长阳路717号,200082
- 折叠
摘要
Abstract
Pretreatraent conditions of detergent method were optimized and contents of NDF (Neutral Detergent Fiber), ADF (Acid Detergent Fiber) and ADL (Acid Detergent Lignin) in tobacco and tobacco products were determined. Results showed; 1) using mixture-solvent (containing benzene, ethanol, and ether) can remove most ester soluble interfering substances in tobacco after 4-hour washing; 2) using ot-amylase can clear up interference of starch during NDF determination; 3) limits of detection of NDF, ADF and ADL are 0.26% , 0.17% , 0.17% , respectively, and the recoveries were all between 89%-101.4% ; 4) differences between contents of NDF and ADF in different types of tobacco leaves are more obvious than in tobacco products; and 5) ADL contents in most tobacco and tobacco products were below 3%.关键词
中性洗涤纤维/酸性洗涤纤维/酸性洗涤木质素/洗涤剂法Key words
neutral detergent fiber/ acid detergent fiber/ acid detergent lignin/ detergent method分类
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杨斌,殷引,张浩博,佘永桢,沈轶..洗涤剂法测定烟草及烟草制品中中性洗涤纤维、酸性洗涤纤维、酸性洗涤木质素的研究[J].中国烟草学报,2012,18(3):10-15,6.
