中国药房2012，Vol.23Issue(18)：1665-1668,4.DOI:10.6039/j.issn.1001-0408.2012.18.14
UPLC-MS法测定人血浆中阿奇霉素的浓度
Determination of Azithromycin Concentration in Human Plasma by UPLC-MS
沈国荣 1董吉 1缪丽燕1
作者信息
- 1. 苏州大学附属第一医院,江苏苏州215006
- 折叠
摘要
Abstract
OBJECTIVE: To establish a method for the determination of azithromycin concentration in human plasma. METHODS: The plasma samples were protein-precipitated, and the samples were determined by UPLC-MS. The determination was performed on Waters Acquity UPLC BEH C18 column with mobile phase consisted of acetonitrile-10 mmol·mL‐1 ammonium formate (66:34) at the flow rate of 0.2 mL·min‐1. The protonated ions of analytes were detected in positive lonization multiple reaction monitoring mode (MRM). The mass transition pairs of m/z 748.36→591.10 and 837.34→158.06 were used to detect azithromycin and roxithromycin. RESULTS: The linear range of azithromycin was 2~1000 ng·mL‐1(r=0.999 5), and the low limit of quantita-tion was 2 ng· mL‐1. The average method recovery rates were 102.6% ~108.0% and average extraction recovery rates were 93.2%~102.2%. The intra-day RSD and inter-day RSD were all less than 8%. CONCLUSION: The method is simple, quick and reliable with high specificity and sensitivity, and it is suitable for the pharmacokinetic study of azithromycin.关键词
阿奇霉素/超高效液相色谱-串联质谱/药动学/血药浓度Key words
Azithromycin/ UPLC-MS/ Pharmacokinetics/ Plasma concentration
分类
医药卫生引用本文复制引用
沈国荣,董吉,缪丽燕..UPLC-MS法测定人血浆中阿奇霉素的浓度[J].中国药房,2012,23(18):1665-1668,4.
